Skp2-P^27kip1通路及其相关凋亡基因在结肠癌组织中的表达及意义  

Expressions of Skp2-P^(27kip1) and apoptosis-related genes in colon carcinoma and significances

作　　者：陈树军[1,2] 郎晓讴[2] 张春阳[2] 张少军[1,2] 姜文华[1] 

机构地区：[1]吉林大学基础医学院组织学与胚胎学教研室,吉林长春130021 [2]沈阳医学院奉天医院普外科,辽宁沈阳110024

出　　处：《吉林大学学报（医学版）》2009年第3期503-506,F0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(20030542-1)

摘　　要：目的:探讨Skp2和P27kip1蛋白在结肠癌中的表达及其相关性,阐明两者在结肠癌的发生和发展中的作用。方法:应用免疫组织化学SABC法检测50例结肠癌患者癌组织和20例正常结肠组织中Skp2、P27kip1、P53和Bcl-2蛋白的表达,比较不同组织中阳性细胞表达百分比,分析其与结肠癌临床病理参数的相关性。结果:与正常组织比较,结肠癌组织中Skp2蛋白阳性表达率增加(P<0.05),P27kip1蛋白阳性表达率降低(P<0.05)。Skp2蛋白阳性表达率在临床Duck’s分期C和D期结肠癌、低分化结肠癌及伴有淋巴结转移的结肠癌组织中增加(P<0.05),P27kip1蛋白阳性表达率在临床Duck’s分期A和B期结肠癌、高分化结肠癌及无淋巴结转移的结肠癌组织中增加(P<0.05)。P53和Bcl-2蛋白在低分化结肠癌组织中均高表达(P<0.05)。结肠癌中Skp2与P27kip1的表达率呈负相关关系(r=-0.282,P<0.05),与P53及Bcl-2蛋白阳性表达率分别呈正相关关系(r1=0.345,r2=0.231,P<0.01)。结论:Skp2和P27kip1蛋白的异常表达参与了结肠癌组织的分化、浸润和转移,相关凋亡基因P53及Bcl-2蛋白表达上调可能是其作用机制之一。Objective To investigate the expression and correlation of Skp2 and p27kipl in colon carcinoma tissue and explore the relationship with the development of colon carcinoma. Methods The protein expressions of Skp2, P^27kip1 P53 and Bcl-2 were detected by immunohistochemistry staining （SABC method） in 50 cases of colon carcinoma tissue （CCT） and 20 normal colonic mucosas （NCM）. The positive expression rates in different tissues were compared and the relationship between the expression and different clinicopathological parameters were analyzed. Results The expression of Skp2 in the CCT was significantly increased compared with NCM （P〈0.05）. The protein expression of P^27kip1 in CCT was significantly lower than that in NCM （P〈0.05）. The expressions of Skp2 were significantly increased in the Duke＇s staging C and D group, lower degree differentiation group and lymph node metastases positive group （P〈0.05）. The expressions of P^27kip1 were significantly increased in the Duke＇ s staging A and B group, high degree differentiation group and lymph node metastases negative group （P〈0.05）. The expressions of P53 and Bel-2 protein were significantly increased in the low degress differentiation CCT （P〈0.05）. The negative correlation was present between Skp2 and P^27kip1 in CCT （r=-0. 282, P〈0.05）, and there were positive correlations between Skp2 and P53, Bcl-2 in CCT （r= 0. 345, r= 0. 231, P（0.05）. Conclusion The abnormal expressions of both Skp2 and P^27kip1 participate in the differentiation, infiltration and metastases of colon carcinoma. It may be realized through up-regulating the expressions of P53 and Bcl-2.

关 键 词：结肠肿瘤 细胞S期激酶相关蛋白2 P^27kip1 蛋白质P53 原癌基因蛋白质C-BCL-2 

分 类 号：R735.35[医药卫生—肿瘤]