IFNγ-基因+874位点单核苷酸多态性与卵巢癌易感性的关系  

Association between single nucleotide polymorphism of interferon-gamma gene +874 site and susceptibility to ovarian cancer

作　　者：武广恒[1] 张家颖[1] 左文静[1] 

机构地区：[1]吉林大学基础医学院生物化学与分子生物学实验中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第3期507-510,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(39870313)

摘　　要：目的:探讨人IFNγ-基因+874位点单核苷酸多态性(SNP)与卵巢癌易感性的关系,为卵巢癌的基因诊断提供依据。方法:采用PCR、克隆和测序技术,检测87例卵巢癌患者(病例组)及86例正常女性(对照组)IFNγ-基因+874位点的基因型。结果:病例组和对照组基因型频数分布均符合Hardy-Weinberg遗传平衡定律(P>0.05);病例组IFNγ-基因+874位点AA、AT和TT基因型频数分布与对照组比较差异无显著性(P>0.05);病例组中IFN-γ基因+874位点T等位基因与A等位基因频数分布与对照组比较差异具有显著性(P<0.05),T等位基因患卵巢癌的风险是A等位基因的1.80倍;TT基因型患卵巢癌的风险是AA型的2.99倍;病例组中上皮性卵巢癌与非上皮性卵巢癌之间基因型频数和等位基因频数分布比较差异均无显著性(P>0.05)。结论:IFNγ-基因+874位点T等位基因可能与卵巢癌的发生有关联,TT基因型可能是罹患卵巢癌易感基因型。Objective To investigate the association between the single nucleotide polymorphism （SNP） of interferon-gamma （IFN-γ） gene ＋874 site and susceptibility to ovarian cancer, and provide a theoretical basis for the gene diagnosis of ovarian cancer. Methods Using the PCR, cloning and sequencing techniques, the genotypes of IFN-γ gene ＋874 site A/T of 87 cases of ovarian cancer patients （case group） and 86 normal women （control group） were detected. Results The distribution of genotypic frequency did not deviate from Hard-Weinberg equilibrium in both case group and control group; the genotypic frequencies of AA, AT and TT had no significant difference between case group and control group （P〉0.05）; the frequencies of IFN-γ gene ＋874 site T and A allele showed significant difference between case group and control group （P〈0.05）, the relative risk suffering from T allele was 1.80 times of A allele; the relative risk suffering from TT genotype was 2. 99 times of AA genotype; the genotypic frequency and allelic frequency had no significant difference between epithelial ovarian cancer and non-epithelial ovarian cancer in case group （P〉0.05）. Conclusion T allele of IFN-γ gene＋ 874 site might have a relationship with generation of ovarian cancer, TT genotype might be susceptibility genotype for ovarian cancer.

关 键 词：卵巢肿瘤 干扰素 基因型 单核苷酸多态性 

分 类 号：Q754[生物学—分子生物学] R737.31[医药卫生—肿瘤]