人肺癌离体组织多环芳烃类DNA加合物的初步研究  

PAH DNA adduct in human lung cancer explants. A preliminary study

作　　者：谢汇江 赵志文[1,2] 王苏民[1,2] 孟庆海 张海青[1,2] 李世业[1,2] 陈肖嘉[1,2] 贺锡文[1,2] 杨柯[1,2] 吕京 崔涛 

机构地区：[1]北京市结核病胸部肿瘤研究所 [2]中国预防医学科学院劳动卫生职业病研究所

出　　处：《中华肿瘤杂志》1998年第3期187-190,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金;北京市自然科学基金

摘　　要：目的探讨某些外在因素和体内多环芳烃类（ＰＡＨ）ＤＮＡ加合物的量以及人肺癌三者的关系，以期评价环境的影响，发现高危人群。方法从动物预初实验到临床病例对照研究，以不同肺疾病行肺切除的患者为对象，采取Ｒａｎｄｅｒａｔｈ的３２Ｐ后标记法。结果测定动物肺标本中的ＰＡＨＤＮＡ加合物的量（０．３９，１．３１，２．８４）与苯并芘染毒剂量（６．２５，２０，４０）之间有较好的剂量反应关系。加合物的量在女性肺癌组与肺良性疾病对照组之间差异有显著性（１３．５９∶７．９０，Ｐ＜０．０５）。女性从事烹调与否与加合物的量亦有明显差异（１２．０１∶６．４１，Ｐ＜０．０３）。另外加合物的量与病理学组织类型、细胞分化程度、淋巴结转移状况有关，如鳞癌明显高于腺癌等。结论ＰＡＨＤＮＡ加合物量高于肺良性病变对照，仅见于女性肺癌；加合物水平随肺癌分化程度下降。Objective To study the influences of external exposure on polycyclic aromatic hydrocarbons(PAH) DNA adduct formation in lung cancer patients. Methods On the basis of pilot experiments in mice, 32 P post labeling assay developed by Randerath was used to measure PAH DNA adduct in human lung tissue explants. Relative adduct label(RAL) value was employed as a measure of adduct formation. A case control study was designed using age , sex , and residence matched patients with lung cancer and benign pulmonary diseases. Results Although the mean RAL value in male lung cancer patients was not significantly different from that in patients with benign diseases, patients who smoked cigarettes had significantly higher RAL value than those who did not smoke(27.62×10 -9 versus 11.42×10 -9 , P =0.0015). In contrast, in female lung cancer patients, the RAL value was significantly higher in patients with lung cancer than in those with benign lung diseases (13.59×10 -9 versus 7.9×10 -9 , P <0.05). Moreover, significant difference in RAL values existed between female smokers and non smokers. Cooking affected female patients only. Higher RAL values were seen in patients with squamous cell carcinoma than with adenocarcinoma ( P <0.05). RAL values were significantly higher in poorly differentiated than in well differentiated lung cancers ( P <0.05). A significant increase in RAL values was observed in patients with lymph node metastasis as compared with those without lymph node metastasis. Conclusion PAH DNA adduct level in lung tissue explants can be taken as a measure of environmental exposure and it correlates with the histological type, degree of differentiation and lymph node metastasis of lung cancer.

关 键 词：肺肿瘤 病理学 DNA加合物 腺癌 鳞状细胞癌 

分 类 号：R734.2[医药卫生—肿瘤]