E-selectin在延迟性异种心脏移植排斥反应中的表达  

Expression of E-selectin in delayed cardiac xenograft rejection

作　　者：唐华[1] 姚榛祥[1] 刘胜春[1] 

机构地区：[1]重庆医科大学附属第一医院器官移植研究室,重庆400016

出　　处：《陕西医学杂志》2009年第6期648-650,共3页Shaanxi Medical Journal

摘　　要：目的:观察小鼠→大鼠异种心脏移植后延迟性排斥反应(DXR)的病理特征,研究E-selectin在异种移植心脏的表达变化,探讨DXR发生机制。方法:袖套法建立小鼠→大鼠颈部异位异种心脏移植模型,将接受异种心脏移植的大鼠随机分为5组,1组(n=16)在供心停跳时取心,余4组(每组n=4)分别在移植术后3、8、16、24 h供心仍跳动时取心,另取未移植小鼠心脏作为对照组(n=4)。各组标本作HE染色、免疫组化SABC法检测E-selectin表达变化。结果:移植心脏HE染色表现为血管内皮损伤、血栓形成、心肌实质损伤、间质出血、炎症细胞浸润等病理特征随移植术后时间逐渐加重,完全排斥时部分心肌凝固性坏死及溶解、广泛间质出血、血管结构崩解及血管内血栓形成、严重炎症细胞浸润。对照组心脏无E-selectin表达,移植组心脏在移植后3 h即有E-selectin表达于血管内皮细胞,且随时间推移表达逐渐增强,排斥时达到高峰。结论:血管内皮细胞激活参与DXR发生,E-selectin表达与DXR发生和发展密切相关,E-selectin表达可早期提示DXR发生。Objective：To study the pathologic features and the expression of E-selectin in delayed cardiac xenograft rejection. Methods：A model of mouse-to-rat cardiac heterotopic xenotransplantation in neck was established by cuff technique. The cardiac xenografts were harvested at 3 h （n= 4）, 8 h （n = 4）, 16 h （n= 4） and 24 h（n=4） after transplantation while the hearts were still beating. Other cardiac hearts（n= 16） were not harvested until rejection time. Untransplanted mouse hearts were studied as controls （n= 4）. All samples were examined by HE and E-selectin expression by immunohistochemistry. Results ： During the period of post- transplantation, the degree of rejection in cardiac xenografts became serious gradually till they were ultimately rejected. Immunohistochemical examination showed that E-selectin expression was found as early as 3 h posttransplantation and increased gradually on vascular endothelial cells of xenografts ,whereas the contral group did ont express. Conclusion： E-selectin expression is related with the development of the delayed xenograft rejection ,and endothelial cells activation involves in also.

关 键 词：心脏移植 移植 异种 移植物排斥 @E-selectin 

分 类 号：R737.14[医药卫生—肿瘤] R654.2[医药卫生—临床医学]