人工液态脂膜法固定鼠肝微粒体  

Immobilization of Rat Liver Microsomes with Liquid Lipid Membrane Encapsulation

作　　者：吕京[1] 谢广云[1] 崔涛[1] 徐立 贺锡雯[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所

出　　处：《卫生研究》1998年第3期149-150,共2页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：报告了用人工液态脂膜包裹法固定鼠肝微粒体并以ＮＡＤＰＨ－细胞色素Ｃ－还原酶为标志酶的初步研究结果，液态脂膜的组成为５６％重石蜡油，３８％轻石蜡油，５％卵磷脂和１％十二烷醇。固定后酶对温度的耐受性下降，而对ｐＨ值的耐受性未见明显变化；固定后酶每单位蛋白的催化活性及在４℃条件下的储存稳定性有所提高。at liver microsomes were immobilized by encapsulating them into liquid lipid membranes(56% paraffin thick,38% paraffin thin,5% lecthin and 1% dodecanol).The resistance against heat and pH profile of the immobilized enzyme NADPH cytochrome C reductase was weakened.However,the activity and storage stability of the immobilized enzyme were enhanced.

关 键 词：人工液态脂膜 微粒体 NADPH 细胞色素C 还原酶 

分 类 号：Q593.1[生物学—生物化学] Q554