中医“肺应秋”理论与大鼠肺脏cAMP、cGMP相关性的实验研究  

Empirical Study on Associativity of cAMP and cGMP with TCM Lung Corresponding with Autumn

作　　者：顾晓静[1] 马淑然[1] 郭霞珍[1] 刘晓燕[1] 袁卫玲[2] 赵树宏[1] 

机构地区：[1]北京中医药大学基础医学院 [2]天津中医药大学基础医学院

出　　处：《山西中医》2009年第6期53-55,共3页Shanxi Journal of Traditional Chinese Medicine

基　　金：国家自然基金资助项目(30672567);教育部科学技术重大研究项目(105030)

摘　　要：目的:探讨中医"肺应秋"与大鼠肺脏第二信使cAMP、cGMP的相关性。方法:采用松果腺摘除动物模型,用ELISA检测四季大鼠肺组织cAMP、cGMP的含量。结果:(1)正常组肺脏中cAMP四季中夏季高于秋季(P<0.05);伪手术组之间均无明显差异;手术组冬季低于春季(P<0.05)、夏季(P<0.01),也低于正常组的冬季(P<0.05)。(2)正常组肺脏中cGMP四季中秋季高于春季(P<0.01);伪手术组秋季高于冬季(P<0.05),冬季低于正常组(P<0.05);手术组夏季高于春季(P<0.01)、冬季(P<0.05),手术组春季低于伪手术组(P<0.05),冬季低于正常组(P<0.05)。结论:(1)肺组织cAMP含量参数依次为:夏>春>冬>秋,cGMP含量参数依次为:春<夏<冬<秋的"肺应秋"剂量相关关系。大鼠秋季肺组织cAMP含量低,cGMP含量高,这可能是"肺应秋"的生理学机制之一。(2)大鼠肺脏cAMP、cGMP含量的季节节律性可能与褪黑素节律性调节有关。Objective ： To investigate the relationship between the theory of TCM Lung corresponding with autumn and the second messenger, cAMP and cGMP, in rats＇lungs. Method： Wistar rats were operated by pinealectomy to make animal models. Then cAMP and cGMP in rats＇lungs were detected with ELISA in four seasons. Result ： ①The level of cGMP in normal group was obviously higher in summer than in autumn （P 〈 0.05 ）. But there was no remarkable difference between normal group and pseudo-operation group. It was lower in operation group in winter than in spring （ P 〈 0.05 ）, summer （ P 〈 0.01 ） and in normal group in winter （ P 〈 0.05 ）. ②The level of cGMP in normal group was higher in autumn than in spring （P 〈 0.01 ）. That in pseudo-operation group was higher in autumn than in winter （P 〈 0.05 ）, and lower in winter than normal group （ P 〈 0.05 ）. That in operation group was higher in summer than in spring （ P 〈 0.01 ） and winter （ P 〈 0.05 ）. And that in operation group in spring was lower than in pseudo-operation group （P 〈 0.05 ） Conclusion： ①Dosage correlativity to the theory of TCM lung , lower in winter than in normal group （ P 〈 0.05 ）. corresponding with autumn is as follows. The content of cAMP in lung is in order of summer 〉 spring 〉 winter 〉 autumn, and the content of cGMP is in order of spring 〈 summer 〈 winter 〈 autumn, cAMP in rats lung in autumn is low, but cGMP is high. This may be one of physiological mechanisms of TCM lung corresponding with autumn. ②The seasonal rhythmicity of cAMP and cGMP in rat lung is related to the seasonal changes of melatonin.

关 键 词：肺应秋 CAMP CGMP 褪黑素 实验研究 

分 类 号：R22[医药卫生—中医基础理论]