维生素D衍生物EB1089对人喉癌细胞Hep-2细胞增殖与凋亡的影响  

Effect of vitamin D analogue EB1089 on proliferation and apoptosis of human laryngeal carcinoma cell line Hep-2

作　　者：卢连军[1] 查定军[1] 薛涛[1] 邱建华[1] 

机构地区：[1]第四军医大学西京医院耳鼻咽喉头颈外科,陕西西安710033

出　　处：《第四军医大学学报》2009年第11期964-966,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨EB1089对人喉癌细胞Hep-2凋亡的作用及可能的机制.方法:MTT法检测不同浓度(0,1,10,100nmol/L)EB1089处理24,48,96h对Hep-2细胞的抑制率;TUNEL检测细胞凋亡;分光光度法检测Caspase-3活性.结果:EB1089在一定浓度范围内,以浓度和时间依赖的方式抑制Hep-2细胞的生长(P<0.05),对Hep-2细胞的抑制率为2.6%～70.0%;10,100nmol/LEB1089处理细胞48h,可诱导Hep-2细胞发生凋亡,其凋亡指数分别为22.8±1.6和53.8±3.6,与对照组相比有统计学差异(P<0.05);EB1089处理细胞12,24,48,96h后Caspase-3的活性增加,与对照组相比分别增加了2.09,2.72,4.91,5.23倍(P<0.05);用Caspase-3活性抑制剂可部分缓解EB1089诱导的Hep-2细胞凋亡.结论:EB1089可能通过诱导Caspase-3活性增加的方式抑制Hep-2细胞增殖,诱导细胞凋亡.AIM： To explore the effect of EB1089 on the apoptosis of human laryngeal carcinoma Hep-2 cells and its mechanism. METHODS： Hep-2 cells were cultured and treated with EB1089 of different concentrations （0, 1,10,100 nmol/L） for 24,48 and 96 h respectively. The growth inhibition rate of Hep-2 cells was detected by MTT method and the cell apoptosis rate was analyzed by TUNEL using fluorescence microscope. Caspase-3 activity was assessed by colorimetric assay. RESULTS： EB1089 inhibited the proliferation of Hep-2 cells in a time- and dose-dependent manner （P 〈 0. 05 ）, with the inhibitory rate ranging from 2. 6% - 70.0%. Forty-eight hours after EB1089 treatment at 10 nmol/L and 100 nmol/L, the apoptosis index of Hep-2 cells was 22. 8±1.6 and 53.8±3.6, respectively. After 10 nmol/L EB1089 treatment for 12,24,48 and 96 h, the activities of caspase-3 were significantly increased respectively by 2. 09, 2. 72, 4.91 and 5.23 folds compared with those in control group （ P 〈 0.05）. The EB1089-induced apoptosis of Hep-2 cell was partly blocked with the treatment of caspase-3 inhibitor, Z-DEVD-FMK. CONCLUSION： EB1089 inhibits the proliferation and induces the apoptosis of Hep-2 cells by increasing caspase-3 activity.

关 键 词：EB1089 喉肿瘤 细胞增殖 细胞凋亡 CASPASE-3 

分 类 号：R739.6[医药卫生—肿瘤]