白眉蝮蛇蛇毒细胞毒素H1对实验性大鼠胃癌抑制作用的实验研究  

Inhibitory effect of cytotoxin from Agkitrodon Acutus venom on gastric cancer

作　　者：鲁珏[1] 徐飞鹏[1] 

机构地区：[1]广东医学院附属医院胃肠外科,广东湛江524000

出　　处：《第四军医大学学报》2009年第11期975-977,共3页Journal of the Fourth Military Medical University

基　　金：湛江市科技公关项目(2008C08006)

摘　　要：目的:从白眉蝮蛇蛇毒中提取细胞毒素H1,观察细胞毒素H1对实验性大鼠胃癌的抑制作用.方法:采用DEAE-Sephadex A-50,TSKgel Pheny柱层析从白眉蝮蛇蛇毒中分离纯化细胞毒素H1.在饮水中添加致癌剂1-甲基-3-硝基-1-亚硝基胍(MNNG),增溶剂吐温20及维生素D3饲喂Wistar大鼠,并应用高张盐水间歇辅以刺激,制造大鼠胃癌模型,将存活的大鼠胃癌模型随机分为阴性对照组、5-FU阳性对照组、细胞毒素H1给药组,连续注射3wk.结果:细胞毒素H1给药组原位肿瘤体积、瘤质量较5-FU组明显降低,具有统计学意义(P<0.05).结论:细胞毒素H1确有抑制实验性大鼠胃癌生长和转移的作用.AIM： To purify cytotoxin from Agkitrodon Acutus venom and to study its inhibitory effect on gastric cancer in rats. METHODS ： Cytotoxin H1 was isolated and purified by successive chromatography on DEAE-Sephadex A-50 and TSKgel Pbeny columns from Agkitrodon acutus venom. To establish the gastric cancer model, the rats were fed with water added N-Methyl-N＇- Nitro-Nitrosoguanidine, Tween 20 and Vitamin D3 and were Stimulated with hypertonicsaline. The established models were randomly divided into negative control group, 5-FU positive control group and treatment group of Cytotoxin H1 and were treated with continuous injection for 3 weeks. RESULTS： In situ tumor volume and tumor weight in treatment group was significantly lower than those in 5-FU group（ P 〈 0.05）. CONCLUSION ： Cytotoxic HI inhibits gastric cancer growth and metastasis in rats.

关 键 词：细胞毒素 白眉蝮蛇毒 大鼠 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]