离子色谱分离-恒电位法联用检测菠菜中硝酸盐含量  

Detection on the Content of Nitrate Ion with Ion Chromatographse Paration-Constant Potential

作　　者：董乐[1,2] 冯珍鸽[2] 王力[2] 

机构地区：[1]泉州师范学院化学与生命科学学院,福建泉州362000 [2]集美大学生物工程学院,福建厦门361021

出　　处：《内蒙古民族大学学报（自然科学版）》2009年第3期263-265,共3页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金项目(20871054);福建省教育厅基金项目(2007F5076);福建省自然科学基金高校专项基金项目(2007J0369)

摘　　要：本文采用离子色谱与恒电位法联用方法对菠菜中硝酸盐进行检测.采用硝酸为流动相,以IonPacAS11-HC阴离子交换柱分离后以恒电位仪为检测器进行安培检测,进样量为25μL时NO3-离子的检出限(以3倍信噪比计)可降至0.2μg/L,痕量NO3-离子标准溶液平行9次进样测定的相对标准偏差为2.5%,经过几百组测试,稳定性好、线性、重现性及灵敏度均令人满意.The nitrate ion determination using ion chromatography has been researched in the paper.With the CHI660 Electrochemical Detector,the Detection Limit of the method（S/N ratio of 3） has reached 0.2μg/L（25μL injected）,using sodium hydroxide as the mobile phase and the AS11-HC as the separate column.The Relative Standard Deviation（RSD） was 2.5%（n=9）.A method of constant potential has been established and compared with the conductivity detector method and with the method of nitrate acid mobile phase.As a result,the constant potential Determination method is better than the suppressed conductivity method and the constant potential method with the nitric acid.

关 键 词：离子色谱 硝酸盐 恒电位法 

分 类 号：Q356.1[生物学—遗传学]