丝光绿蝇抗菌物质在不同pH下抑菌活性的研究  

A study on antibacterial activity of antibacterial substance induced by Lucilia sericata at different pH conditions

作　　者：许兵红[1] 王东[1] 朱国勇[1] 王歌[1] 

机构地区：[1]新乡医学院寄生虫学教研室,453003

出　　处：《国际医学寄生虫病杂志》2009年第3期155-158,共4页International JOurnal of Medical Parasitic Diseases

基　　金：河南省医学科技创新人才工程资助项目（2003-50）

摘　　要：目的探讨丝光绿蝇经针刺体壁诱导产生的抗菌物质在不同的pH条件下的抑菌活性。方法丝光绿蝇三龄幼虫经针刺体壁诱导后48h，提取其血淋巴，观察其在不同的pH琼脂平板上对溶壁微球菌和金黄色葡萄球菌的抑菌效果；经不同pH缓冲液处理后，观察其对溶壁微球菌的抑菌效果。结果丝光绿蝇幼虫经针刺诱导后48h的血淋巴，在不同pH琼脂平板上对金黄色葡萄球菌的抑菌效果，pH6．0、6．5环较大、较清晰，随pH增加而环直径减小。对溶壁微球菌的抑菌效果，在pH6．0时，抑菌环直径最大，环直径随pH增加而减小。用不同pH的缓冲液稀释处理时，pH6．0缓冲液稀释后对溶壁微球菌的抑菌环最大，pH9．0缓冲液稀释后对溶壁微球菌的抑菌环最小。结论丝光绿蝇经针刺体壁诱导产生的抗菌物质在酸性条件下比在碱性条件下具有更强的抑菌活性。Objective To investigate the antibacterial activity of antibacterial substance in Lucilia sericata induced by pricking the skin of maggots （fly＇s larvae） at different pH conditions. Methods The hemolymph from the third instar maggots was collected 48 h after pricking, and the antibacterial effect was tested against Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions. The antibacterial effect against M. lysodeikticus was also detected after the hemolymph was diluted by different pH buffer solutions. The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M. lysodeikticus and S. aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates. In the group diluted by pH 6.0 buffer solutions, the antibacterial ring of the maggot hemolymph against M. lysodeikticus was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.

关 键 词：丝光绿蝇 抗菌物质 诱导 PH 

分 类 号：R384.2[医药卫生—医学寄生虫学] R914.5[医药卫生—基础医学]