MCF-7细胞株中SP与非SP细胞特点比较研究  

Characteristics of side population cells and non-side population cells from MCF-7

作　　者：王曦[1] 金从国[2] 邹天宁[1] 陈文林[1] 王龙强[1] 

机构地区：[1]昆明医学院第三附属医院云南省肿瘤医院乳腺癌临床研究中心,云南昆明650018 [2]昆明医学院第三附属医院云南省肿瘤医院肿瘤研究所,云南昆明650018

出　　处：《中华肿瘤防治杂志》2009年第9期657-660,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：昆明医学院研究生创新基金(2007207)

摘　　要：目的:研究乳腺癌MCF-7细胞系中SP细胞与非SP细胞表面标记的特点,并进行比较分析。方法:以体外培养的MCF-7细胞作为研究对象,采用Hoechst33342染色,并用流式细胞仪分选出SP细胞和非SP细胞,检测两种细胞的各种表面蛋白或抗原和细胞周期增殖状况。结果:乳腺癌MCF-7细胞株中SP细胞的含量为(10.1±0.84)%;分选后SP细胞的CD38、CD117和CD34表达率分别为(1.67±0.60)%、(0.43±0.22)%和(0.27±0.12)%,非SP细胞分别为(1.37±0.49)%、(0.42±0.24)%和(0.33±0.15)%,差异无统计学意义,P均>0.05;两者CD133、CD24、ABCG2的表达率分别为(34.33±7.03)%、(46.68±4.44)%、(85.82±2.48)%和(5.62±1.16)%、(75.38±5.14)%、(4.89±1.80)%,差异有统计学意义,P均<0.05;两者细胞中G0/G1、S、G2/M期细胞数分别为(81.34±6.69)%、(13.48±5.90)%、(5.18±2.42)%和(57.71±6.46)%、(23.14±6.46)%,(19.14±4.61)%,差异有统计学意义,P均<0.05;两者Ki-67表达率分别为(12.63±2.10)%、(34.37±4.71)%,差异有统计学意义,P=0.000。结论:乳腺癌MCF-7细胞系中含有比例较高的SP细胞,且SP与非SP细胞有可以分别鉴定和纯化的抗原差别。选择SP方法分离乳腺癌干细胞具有科学性。OBJECTIVE： To investigate the markers between side population ceils and non-side population cells from MCF-7 cancer cell line. METHODS： After staining with Hoechst33342 dye, side population ceils and non-side population cells were sorted by FACS from MCF-7, and these cell markers, antigens and cycles were measured. RESULTS： The side population ceils containing （10. 1 ± 0.84）% existed in MCF-7. The proportions of CD38, CDl17, CD34 were （1.67 ± 0.60）%, （0.43±0.22）%, （0.27±0.12）% and （1.37± 0.49）%, （0.42±0.24）%, （0. 33±0.15）% in side population cells and non-side population cells, respectively. There was no marked difference （P〉 0.05）. The proportions of CD133,CD24, ABCG2 were （34.33± 7.03）% , （46.68 ± 4.44）%, （85.82±2.48）% and （5. 62±1.16）%, （75.38± 5.14） %, （4.89 ± 1.80） % in side population cells and nonside population cells, respectively. There was significant difference （P〈0.05）. The proportions of G0/G1 ,S, G2/M, Ki-67 were （81.34±6.69）%, （13. 48 ± 5. 90） % , （5.18± 2.42）%, （12.63±2.10）% and （57.71±6.46）%, （23.14±6.46）%,（19.14±4.61）% , （34. 37±4. 71） % in side population cells and non-side population cells, respectively. There was significant difference （P =0. 000）. CONCLUSIONS： Side population cells express high level in breast cancer cell line, and SP and non-SP cells can be identified and purified by using their antigenic markers properties. SP may be a scientific method to isolate breast cancer stem cells.

关 键 词：乳腺肿瘤 干细胞 细胞 培养的 

分 类 号：R737.9[医药卫生—肿瘤]