血管抑素基因联合5-FU腹腔化疗治疗大肠癌术后腹腔种植的实验研究  

Combination of angiostatin gene therapy and peritoneal chemotherapy with 5-FU to treat peritoneal implantation of colonic carcinoma

作　　者：黄春锦[1] 沈达明[1] 杜卫东 袁祖荣[1] 乔伟伟[3] 唐健雄[1] 程爱群[1] 张赣生[4] 王一倩[4] 于晓峰[4] 竺越[4] 

机构地区：[1]上海市华东医院普外科,上海200040 [2]Cancer Research Center,Sunnybrook Health Center,Institute of Medical Sciences,University of Toronto [3]复旦大学动物实验部,上海200040 [4]上海市华东医院消化科,上海200040

出　　处：《中华肿瘤防治杂志》2009年第9期661-663,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:观察血管抑素基因联合5-氟尿嘧啶(5-FU)腹腔化疗对预防大肠癌术后腹腔种植的作用。方法:RT-PCR扩增血管抑素基因,构建重组腺病毒载体。建立人结肠癌LoVo细胞腹腔种植的模型,给予血管抑素基因联合5-FU腹腔化疗,并观察其对大肠癌细胞腹腔种植的影响。结果:各组裸鼠的致瘤率和死亡率为100%。血管抑素基因联合5-FU腹腔化疗治疗组的腹水出现时间晚〔(22.3±5.4)d〕,荷瘤生存时间长〔(47.3±8.4)d〕,治疗组肿瘤组织中血管抑素基因表达为阳性,肿瘤微血管密度(3.64±0.58)明显低于其他各组。结论:血管抑素的基因治疗与传统的腹腔化疗联合应用,可能是预防大肠癌术后腹腔种植的一种有效方法。OBJECTIVE： To study the thereopeutic effect of combination of angiostatin gene therapy and peritoneal chemothera py with 5 FU to peritoneal implantation of colonic carcinoma. METHODS： Angiostatin gene was cloned into adenovirus vector. LoVo cells inplanting model was built, which were treated with combining angiostatin gene therapy with peritoneal chemotherapy with 5-FU or other therapy. RESULTS：Both the rates of sickness and the rate of death were 100%. The survival time in the group of the combination of angiostatin gene therapy and peritoneal chemotherapy with 5 FU [（47.3±8.4） d] was much longer than that in other groups. And ascites was found late [（22.3±5.4） d]. There were much less microvessels（3.64± 0.58）and stronger angiostatin gene expression in tumor tissues in the treatment group. CONCLUSION： It may be a good method to treat peritoneal implantation of colonic carcinoma with angiostatin gene therapy combinined with peritoneal chemotherapy with 5-FU.

关 键 词：血管抑制素类 结直肠肿瘤 氟尿嘧啶/治疗应用 注射 腹腔内 

分 类 号：R735.34[医药卫生—肿瘤]