含HN和VP3及IL-18重组鸡痘病毒体内抑制骨肉瘤细胞S-180效应的观察  

Therapeuti effects of recombinant fowl-pox viruse including HN,VP3 and IL-18 on mice bearing S-180 tumor

作　　者：张治宇[1] 蔡郑东[1] 李宵[2] 华莹奇[1] 杨成为[1] 李国东[1] 孙伟[1] 

机构地区：[1]第二军医大学附属长海医院骨科,上海200433 [2]军事医学科学院动物病毒研究所,吉林长春130062

出　　处：《中华肿瘤防治杂志》2009年第9期664-667,672,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究含凋亡素基因、鸡新城疫病毒的HN基因及IL-18基因的重组鸡痘病毒对小鼠骨肉瘤的抑制效应。方法:建立BALB/C小鼠荷S-180骨肉瘤模型,瘤内注射重组鸡痘病毒,测定肿瘤生长率、抑瘤率和小鼠血清唾液酸含量,肿瘤组织病理学分析检测重组鸡痘病毒对骨肉瘤的抑制效应。结果:rFPVHN、rFPVVP3和rFPV3(rFPVVP3IL-18)均对骨肉瘤有抑制作用,但rFPV3组抑瘤率高于rFPVHN组、rFPVVP3组和鸡痘病毒野毒对照组达到51.7%,血清唾液酸含量明显减低(4.22±0.27),P=0.008 7。结论:凋亡素基因、鸡新城疫病毒的HN基因及IL-18基因联合应用具有协同抑制骨肉瘤的效应。OBJECTIVE： To explore the effective method of tumor gene therapy and studying the antitumor effect of HN gene, VP3 gene and IL-18 gene. METHODS： The osteosareoma model in BALB/c mice was constructed and the rFPV were injected into the tumor. The therapeutic effect of rFPV to BALB/e mice bearing S- 180 tumor was observed, and the rate of tumor growth, rate of tumor suppression, sialic-acid in serum, and pathology analysis were detected by immunohistochemistry. RESULTS： Compared with the control groups （rFPVHN group and rFPVVP3 group）, the tumors in the group of rFPV3 （rFPVVP3 IL -18） were decreased obviously, and the rate of tumor suppression reached 51.7 %. The sialic-acid in serum obviously decreased （4. 22 ± 0.27 ）, P = 0. 008 7. CONCLUSIONS： The rFPV3 is able to suppress the proliferation of the tumor cells in BALB/ c mice bearing S-180 tumor. HN gene, VP3gene and IL-18 gene have synergistic inhibition effect on osteosareoma.

关 键 词：鸡痘病毒 骨肉瘤 基因 肿瘤抑制 

分 类 号：R738.1[医药卫生—肿瘤]