改构苹果多糖对人结肠癌HT29细胞凋亡的影响  

Effects of Modified Apple Polysaccharides on the Apoptosis of Human Colon Cancer HT29 cells

作　　者：伍焕杰[1] 甘洪全[1] 李宇华[1] 牛银波[2] 孙阳[1] 刘莉[1] 梅其炳[1] 

机构地区：[1]第四军医大学药学系药理教研室,陕西西安710032 [2]西北工业大学生命科学院,陕西西安710072

出　　处：《现代生物医学进展》2009年第11期2080-2082,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨改构苹果多糖(modified apple polysaccharides,MAP)对人结肠癌HT29细胞凋亡的影响及其机制。方法:采用MTT法观察不同浓度MAP对HT29细胞增殖的影响;TUNEL法和流式细胞术研究MAP对HT29细胞凋亡的作用;Western Blot法检测给予不同浓度MAP后,HT29细胞中凋亡相关蛋白caspase3,8,9、Bax和Bcl-2表达的变化。结果:与对照组相比,MAP对HT29细胞增殖有明显的抑制作用且能诱导HT29细胞的凋亡;MAP可促进HT29细胞caspase3,8,9及Bax的表达,降低Bcl-2的表达,且均呈剂量依赖性。结论:MAP可以通过内部和外部途径诱导HT29细胞凋亡。Objective： To investigate the effects of modified apple polysaccharides （MAP） on the apoptosis of human colon cancer HT29 cells and the mechanisms. Methods： The effects of different doses of MAP on proliferation of HT29 cells were observed by MTT assay, and the effects of MAP on the apoptosis of HT29 cells were investigated by TUNEL assay and flow cytornetric analysis. And Western Blot was employed to determine the expressions ofcaspase 3, 8, 9, Bax and Bcl-2 of liT29 cells after given MAP. Results： MAP can inhibit proliferation of HT29 cells significantly in a dose-dependent manner in contract to control. And MAP can also induce the apoptosis of HT29 cells, increase the expression ofcaspase 3, 8, 9 and Bax and decrease the expression of Bcl-2 in HT29 cells dose-dependently. Conclusions： MAP could induce apoptosis of HT29 cells. And it seems that MAP exerts this function through both the intrinsic and extrinsic ways ofapoptosis.

关 键 词：改构苹果多糖 结肠癌 细胞凋亡 

分 类 号：R735.35[医药卫生—肿瘤]