α1-肾上腺素受体对胆管癌细胞增殖的影响  

Effects of α1-adrenergic receptor on the proliferation of cholangiocarcinoma cells

作　　者：黄竹[1] 刘子沛[1] 夏锋[1] 海军[1] 邓小明[1] 王曙光[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所、中国人民解放军西南肝胆外科医院,重庆400038

出　　处：《中华消化外科杂志》2009年第3期193-196,共4页Chinese Journal of Digestive Surgery

基　　金：国家自然科学基金面上项目（30772109）

摘　　要：目的探讨α1-肾上腺素受体对胆管癌细胞增殖的影响。方法应用SABC免疫组织化学法检测2002年8月至2008年3月于西南医院行手术切除的36例人胆管癌组织和4例正常胆管组织中α1-肾上腺素受体的表达，并用去甲肾上腺素（NE）以及受体阻滞剂酚妥拉明、哌唑嗪处理胆管癌细胞，观察细胞的增殖变化。采用χ^2检验分析结果。结果发生胆管癌淋巴结转移的患者中α1-肾上腺素受体强阳性表达率为68％（17／25），明显高于未转移患者的9％（1／11），两者比较差异有统计学意义（χ^2=10．604，P〈0．05），且胆总管中、下段癌的α1-肾上腺素受体强阳性表达率为85％（11／13），高于肝门部胆管癌的30％（7／23），两者比较差异有统计学意义（χ^2=9．753，P〈0．05）。NE促进胆管癌细胞的增殖，此作用呈浓度依赖性，随着时间的延长，促增殖作用逐渐减弱。该作用由α1-肾上腺素受体介导，在非选择性α1-肾上腺素受体阻滞剂酚妥拉明及选择性α1-肾上腺素受体阻滞剂（哌唑嗪）存在的情况下可消除NE对胆管癌细胞的促增殖作用。结论α1-肾上腺素受体的表达受淋巴结转移以及胆管癌部位影响，呈强阳性表达的α1-肾上腺素受体参与了胆管癌细胞增殖及转移过程。Objective To investigate the correlation between α1-adrenergic receptor and the pathological behavior of cholangiocarcinoma, and the effects of norepinephrine （NE） on the proliferation of cholangiocarcinoma cell line QBC939. Methods Thirty-six samples of cholangiocarcinoma were resected in Southwest Hospital from August 2002 to March 2008. The expression of α1-adrenergic receptor in the 36 samples of cholangiocarcinoma tissue and 4 samples of normal bile duct tissue were detected by SABC technique. The proliferation of cholangiocarcinoma cell line QBC939 was detected after processing the cells with NE, phentolamine and prazosin. All the data were analyzed by chi-sqnare test. Results The high positive expression rate of α1-adrenergic receptor was 68% （17/25） in patients with lymph node metastasis, which was significantly higher than 9% （1/11 ） in patients without lymph node metastasis （ χ^2 = 10. 604, P 〈 0.05 ）. The high positive expression rate of α1-adrenergic receptor was 85% （ 11/13 ） in patients with middle and low positioned cholangiocarcinoma, which was significantly higher than 30% （7/23） in patients with hilar cholangiocarcinoma （ χ^2 = 9. 753, P 〈 0.05 ）. NE promoted the proliferation of cholangiocarcinoma cell line QBC939 by stimulating the expression of α1-adrenergic receptor, and in a concentration-dependent manner. The proliferative effect was weakened as time passed by, and it was eliminated by phentolamine and prazosin. Conclusions The expression of α1-adrenergic receptor is diverse due to lymph node metastasis and the location of the tumor, α1-adrenergic receptor with high expression may play an important role in the proliferation and metastasis of cholangiocarcinoma.

关 键 词：Α1-肾上腺素受体 胆管肿瘤 转移 细胞增殖 

分 类 号：R735.8[医药卫生—肿瘤]