组织特异性胞嘧啶脱氨酶和5-氟胞嘧啶热化疗对裸鼠结肠癌肝脏转移的影响  

Effects of tissue specific cytosine deaminase/5-fluorocytosine thermotherapy on hepatic metastasis of colonic carcinoma in nude mice

作　　者：黎成金[1] 王羊[1] 王烈[1] 涂小煌[1] 王瑜[1] 张宝明[1] 

机构地区：[1]南京军区福州总医院南京军区普通外科研究所,福州350025

出　　处：《中华消化外科杂志》2009年第3期213-216,共4页Chinese Journal of Digestive Surgery

基　　金：福建省自然科学基金（C0410045）

摘　　要：目的探讨组织特异性胞嘧啶脱氨酶／5-氟胞嘧啶（CD／5-FC）系统热化疗对裸鼠结肠癌肝脏转移的影响。方法将45只裸鼠按随机数字表法分为3组：对照组、非热疗组、热疗组，每组15只。门静脉注射法建立结肠癌肝脏转移动物模型，3组分别给予不同的治疗方法。采用χ^2检验和单因素方差分析3组肝脏肿瘤转移率、转移数目；观察各组病理学变化、肿瘤细胞凋亡指数；荧光定量RT-PCR和Western blot检测肿瘤组织中CD基因的表达情况。结果对照组、非热疗组、热疗组肝脏平均转移癌结节数目和转移率分别为（4．6±1．3）、（2．2±1．0）、（0．5±0．8）个和100．0％、60．0％、13．3％，3组比较差异有统计学意义（F=25．898，χ^2=5．208，19．548，5．168，P〈0．05）；肿瘤细胞凋亡指数平均为4．6％、9．9％和17．4％。热疗组可见大量细胞空泡变性、坏死、溶解现象，有较多的凋亡小体形成。3组均可以检测到CD基因的表达。结论组织特异性CD／5-FC系统热化疗对转CD基因结肠癌LoVo细胞裸鼠肝脏转移有明显的抑制作用。Objective To investigate the effects of tissue specific cytosine deaminase/5-fluorocytosine （CD/5-FC） thermotherapy on hepatic metastasis of colonic carcinoma in nude mice. Methods Forty-five nude mice were randomly divided into control group, 5-FC group and 5-FC thermotherapy group according to the random number table （ 15 mice in each group）. Mice models of hepatic metastasis of colonic carcinoma were established by portal vein injection of LoVo/CEACD cells. The hepatic metastasis rate and number of metastatic nodules of the 3 groups were compared by chi-square test and one-way ANOVA. The pathological changes in tumor tissues and apoptotie index of tumor cells were observed. The expression of the CD gene in tumor tissues was detected by fluorescent quantitative RT-PCR and Western blot. Results The number of metastatic nodules and liver metastasis rate were 4.6 ± 1.3 and 100.0% in control group, 2.2 ± 1.0 and 60.0% in 5-FC group, 0.5 ± 0.8 and 13.3% in 5-FC thermotherapy group, with statistical difference among the 3 groups （ F = 25. 898, χ^2 = 5. 208, 19.548, 5. 168, P 〈0.05）. The mean apoptotic indexes of tumor cells of the 3 groups were 4.6% , 9.9% and 17.4% , respectively. Vacuolar degeneration, cell necrosis, cytolysis and apoptotic bodies were mostly observed in the 5-FC thermotherapy group. The expression of CD gene in tumor tissue was detected in all the groups. Conclusion Tissue specific CD/5-FC thermotherapy has inhibitory effects on the hepatic metastasis of LoVo cells transfected with CD gene.

关 键 词：结肠肿瘤 肝脏转移 裸鼠 胞嘧啶脱氨酶 5-氟胞嘧啶 基因治疗 

分 类 号：R735.35[医药卫生—肿瘤]