分光光度法测定蒙古栎叶中多酚的含量  

Spectrophotometric determination of polyphenols in Quercus mongolicus Fisch leaves by means of Folin-ciocalteu reagent

作　　者：张国友[1,2] 唐玲[1,2] 陈玮[1] 何兴元[1] 黄伟[1,2] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]中国科学院研究生院,北京100049

出　　处：《中国科学院研究生院学报》2009年第3期319-322,共4页Journal of the Graduate School of the Chinese Academy of Sciences

基　　金：国家自然科学基金重点项目(90411019)资助

摘　　要：以没食子酸为标准品,使用福林酚试剂(FC),通过分光光度法,对蒙古栎树叶中植物多酚含量进行了测定.首先从方法的稳定性、线性关系、精确性和准确性角度,对FC法进行了调整,确定了以没食子酸为标准的最佳反应条件.结果表明,最佳反应条件为0.15mL FC试剂(1mol/L),0.15mL 10%体积分数的Na2CO3,25℃反应80min后于760nm处测吸光值.使用调整后的方法测得结果为:蒙古栎叶中总酚含量为6.39%,RSD为1.90%.方法操作简单,结果重现性好,可用来测定蒙古栎叶中总酚的含量.The total polyphenols（TP）content of Quercus mongolicus Fisch leaves extracts was analyzed by Folinciocalteu colorimetry, with gallic acid as standard. The method was improved and verified in the aspects of stability, linearity, precision and accuracy. The results showed that the total polyphenols content of Quercus mongolicus Fisch leaves extracts could be well calculated according to their colorimetric absorption at 760nm by applying Folinciocalteu reagent （lmol/L） 0. 15mL and 10% volume fraction of Na2CO3 0. 15mL at 25℃ for 80min. The （TP） content in Querous mongolicus Fisch leaves determinated by the method was 6.39 % and RSD was 1.90%.

关 键 词：蒙古栎 多酚 福林酚法 分光光度法 

分 类 号：Q-03[生物学]