大豆异黄酮对大鼠内分泌干扰作用研究  

Study on Endocvine Disruptor Effects in the rat of Soy Isoflavones

作　　者：张文众[1] 王伟[1] 张晓鹏[1] 宋雁[1] 刘兆平[1] 李宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2009年第3期228-231,共4页Chinese Journal of Food Hygiene

基　　金：国家"十五"科技攻关重大项目(2006BAK02A07)

摘　　要：目的探索大豆异黄酮(SIF)经口摄入后,在体内的抗雄激素作用机制和剂量-反应关系。方法选用4～5周雄性SD大鼠做HERSHBERGER试验,分别经灌胃给与0.2、0.5、1.5和4.5 g/kgBW的SIF,以3.0 mg/kgBW的氟他胺为阳性对照,0.4 mg/kg睾酮对照(TP组)和空白对照组,除空白对照其余各组均给予睾酮,所有剂量组连续灌胃10天。试验结束称量腹侧前列腺、精囊和凝结腺、肛提肌加球海绵体肌、阴茎头和尿道球腺重量,并测定促黄体激素和睾酮。结果和TP组比较,大豆异黄酮0.50,1.50和4.5 g/kgBW剂量组的体重和增重明显降低(P<0.05),1.50和4.5 g/kgBW剂量组的阴茎头/肾脏和尿道球腺/肾脏的比值降低(P<0.05),4.5 g/kgBW剂量组的肛提肌/肾脏比值降低(P<0.05),1.5 g/kgBW组的LH/T比值升高(P<0.05)。结论大剂量(1.5 g/kgBW和更高)的大豆异黄酮经口摄入产生抗雄激素作用,这种抗雄激素作用可能是一种间接效应。Objective To investigate the androgen antagonists mechanisms in the rat of soy isoflavones in vivo, and also to study the relationship of dose-response. Method HERSHBERGER assay was carried out in immature male SD rats at the age of 4-5 weeks old, 0.2, 0.5, 1.5 and 4.5 g/kg soy isoflavones, and 3.0 mg/kg flutamide as positive control, 0.4 mg/kg Testosterone Propionate （TP）. which was given to every groups, as negative control, vehicle as blank control, all groups were administered orally for 10 consecutive day. In the end of test, animals were anesthetized, weight of ventral Prostate, seminal vesicles together with coagulating gland, ]evator ani bulbocavernous muscles, glans penis, bulbeurethral glands were measured, testosterone （T） and luteinizing hormone （LH） levels were measured. Results In compariton with TP control, body weight decreased in the groups of 0.50, 1.50 and 4.5 g/kg of soy isoflavones. The weights of glans penis and bulbourethral glands weights were found to be significantly decreased in the groups of 1.50 and 4.5 g/kg soy isoflavones （ P 〈 0.05）. The weights of levator ani muscles decreased in the group of 4.5 g/kg soy isoflavones （ P 〈 0.05） ; LH and ratio of LH/T increase in the group of 1.5 g/kg soy isoflavones （ P 〈 0.05）. Conclusion At the level of high dose soy isoflavanes had androgen antagonist effects in vivo, and the effect may be caused indirectly.

关 键 词：异黄酮类 激素拮抗药 内分泌干扰物 

分 类 号：R151[医药卫生—营养与食品卫生学]