索拉非尼联合三氧化二砷对肝癌细胞移植瘤和血管生成抑制作用的探讨  

Sorafenib combined with Arsenic trioxide inhibiting hepatocellular carcinoma xenografts and angiogenesis in nude mice

作　　者：张华[1] 罗荣城[2] 伍婧[3] 

机构地区：[1]广东省人民医院肿瘤中心肿瘤内科广东省医学科学院,广东广州510080 [2]南方医科大学南方医院肿瘤中心,广东广州510515 [3]佛山市第一人民医院肿瘤内科,广东佛山528000

出　　处：《中华肿瘤防治杂志》2009年第8期578-582,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:检测索拉非尼(Sorafenib)单药、三氧化二砷(As2O3)单药以及两药联合对肝癌细胞移植瘤和血管生成抑制作用,探讨两药联用的协同抗肿瘤机制。方法:BALB/C裸鼠皮下接种肝癌HepG2细胞,成瘤后随机分成Sorafenib、As2O3单药组和Sorafenib+As2O3联合用药组,另设不加药的对照组,观察各组肿瘤生长情况,免疫组织化学法检测各组移植瘤组织的微血管密度(MVD)及VEGF表达情况。结果:与对照组比较,单药Sor-afenib、As2O3组及联合组的瘤体积和瘤质量均显著减少,P值均为0.000;联合组的瘤体积和瘤质量较单药Sorafenib及As2O3组显著减少,P<0.05。As2O3和Sorafenib组的血管密度较对照组减少,P值均为0.000;联合用药组的血管密度较As2O3和Sorafenib组减少,P值均为0.000。与对照组相比,Sorafenib、As2O3单药组和联合组VEGF表达明显减少,P值分别为0.024、0.039和0.002;与Sorafenib和As2O3单药组相比,联合组VEGF的表达下降更明显,P值分别为0.037和0.028。结论:Sorafenib及As2O3协同抑制肝癌细胞移植瘤的生长和血管生成。OBJECTIVE： To investigate the inhibitory effect of Sorafenib in combination with Arsenic trioxide （As2O3） on hepatocellutar carcinoma xenografts and its relationship of angiogenesis. METHODS： Construct nude mouse tumor animal model. The nude mouse with HepG2 cell xenografts were random ized into control group, As2O3-treated group, Sorafenib-treated group and combination treated group. VEGF protein expressions and micro-vessel density （MVD） were assessed by immunohisto chemistry. RESULTS： The tumor volume and weight of Sorafenib and As2 03 alone groups were lower than those in the control groups after treatments, P= 0. 000. The tumor volume and weight of combination group was lower than those in the Sorafenib and As2Oa alone groups, P〈0.05. The MVD were decreased in the three groups compared with control group （P=0. 000）. The one of combination was more obvious compared with Sorafenib and As2O3 alone groups （P〈0. 000）. The expressions of VEGF were obviously decreased in Sorafenib and As2O3 alone and in combination groups （P=0. 024; P=0. 039; P=0. 002）, The one of combination was more obvious compared with Sorafenib and As203 alone groups （P=0. 037; P=0. 028）. CONCLUSION： Sorafenib in combination with As2O3 can inhib ire the proliferation of hepatocellular carcinoma xenografts and angiogenesis in nude mice.

关 键 词：肝肿瘤 索拉非尼 三氧化二砷 新生血管化 病理性 

分 类 号：R735.7[医药卫生—肿瘤]