活版印刷生物芯片原位合成仪的研制  

Research and development of biochip in situ synthesizer based on typography principle

作　　者：汤建新[1,2] 何建迪[1] 赵于前[1] 

机构地区：[1]中南大学信息物理工程学院,长沙410083 [2]湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室,株洲412006

出　　处：《仪器仪表学报》2009年第5期905-910,共6页Chinese Journal of Scientific Instrument

基　　金：国家自然科学基金(60571001);国家863计划(2006AA03Z357);中国博士后科学基金(20060390887,20070420826);湖南省自然科学基金(09JJ3119)资助项目

摘　　要：研究开发了一种基于活版印刷原理的生物芯片原位合成仪。该仪器通过设计友好的触摸屏人机界面对系统进行操作、采用可编程序控制器(PLC)实现基因芯片和多肽芯片合成过程的定位压印和液体传输控制;详细描述了仪器的组成:压印平台、精密印刷模版、平面定位系统、机械手、液气传输系统和控制系统;最后在三坐标仪下测量了该仪器的控制精度,测试结果表明,系统定位精度为28.4μm、重复精度为16μm,印刷模版精度为7μm,能实现高精度定位压印,满足活版印刷原位合成生物芯片的要求。A novel machine for in situ synthesis of biochip was developed based on the principle of typography printing. The friendly touch-sensitive screen human-machine interface （HMI） was designed to manipulate the apparatus. The programmable logic controller was employed to control the operation steps involving in biochip synthesis, including locating and imprinting, and delivering the specific liquid. The structure of the machine is described here in detail, which consists of the platform for imprinting, precision printing stencil, one-way positioning device, manipulator, and liquid ＆ gas delivery system and control system. Finally, the operation accuracy of the machine was tested using three-coordinated measurement instrument： the positioning accuracy is 28.4 μm, repeatability accuracy of printing 16 μm, and accuracy of printing stencil 7 μm. The test results show that the accuracy of the machine is good enough to meet the demand of in situ synthesis of biochip based on the principle of typography printing.

关 键 词：活版印刷 原位合成 生物芯片 合成仪 

分 类 号：TM24[一般工业技术—材料科学与工程]