氯霉素全抗原的合成与鉴定  

Synthesis and Identification of Complete Antigen of Chloramphenicol

作　　者：董斌[1] 袁茵[1] 段涛[1] 林月霞[1] 田素娟[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510006

出　　处：《食品科学》2009年第11期163-165,共3页Food Science

基　　金：广东省科技攻关计划项目(2006B20501005);广东省医学科学技术研究基金项目(A2006323);广州市科技计划项目(2006JI-C0221)

摘　　要：目的:为获得较高偶联率氯霉素全抗原,探索和优化碳二亚胺法(EDC)合成氯霉素(CAP)与牛血清白蛋白(BSA)全抗原合成条件。方法:采用单因素设计,优化影响合成的主要因素:蛋白浓度、CAP与BSA的摩尔比例、反应体系pH值,通过紫外法和三硝基苯磺酸(TNBS)法测定合成后的偶联率。结果:EDC法合成氯霉素全抗原较优条件为BSA浓度15mg/ml、CAP:BSA=70:1、pH7.4,可得到偶联率为15左右的氯霉素全抗原。结论:EDC法可用于制备较高偶联率的氯霉素全抗原,以用来进行抗体制备。Objective： To prepare a complete antigen of chloramphenicol （CAP） with high coupling rate, and optimize the coupling conditions of CAP and bovine serum albumin （BSA） by carbodiimide method. Method： Single-factor tests were designed to optimize three main factors affecting the synthesis such as protein concentration, mole ratio of CAP to BSA and pH in reaction system, and ultraviolet （UV） spectroscopy and TNBS method were employed to determine the coupling rate after the synthesis. Results： The optimum BSA concentration, mole ratio of CAP to BSA and pH were 15 mg/ml, 70：1 and 7.4, respectively. Under these conditions, the coupling rate of CPA antigen was about 15. Conclusion： The complete CPA antigen with high coupling rate can be prepared by carbodiimide method for the preparation of antibody.

关 键 词：氯霉素 牛血清白蛋白 碳二亚胺法 偶联 

分 类 号：S859.79[农业科学—临床兽医学]