Responses of wheat seedlings to cadmium,mercury and trichlorobenzene stresses  

作　　者：GE Cailin DING Yan WANG Zegang WAN Dingzhen WANG Yulong SHANG Qi LUO Shishi 

机构地区：[1]Jiangsu Provencial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China [2]College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China

出　　处：《Journal of Environmental Sciences》2009年第6期806-813,共8页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30300026);the Application of Nuclear Techniques in Agriculture from the Chinese Ministry of Agriculture (No. 200803034)

摘　　要：The molecular response of wheat（Triticum aestivum L.,cv.Yangmai 13） seedlings to heavy metal（Cd,Hg） and 1,2,4-trichlorobenzene（TCB） stresses were examined by two-dimensional gel electrophoresis,image analysis,and peptide mass fingerprinting.The results showed inhibitions of root and shoot growth by Cd,Hg,and TCB.These stresses led to water deficit and lipid phosphorylation in the seedling which also promoted protein phophorylation in the leaves.Hg stress inhibited protein synthesis while Cd and TCB stresses induced or up-regulated more proteins in the leaves.Most of these induced proteins played important roles in the biochemical reactions involved in tolerance of wheat to Cd and TCB stresses.The primary functions of Cd-and TCB-induced proteins included methionine metabolism,Rubisco modification,protein phosphorylation regulation,protein configuration protection,H＋ transmembrane transportation and also the synthesis of ethylene,defense substances and cell wall compounds.The molecular response of wheat（Triticum aestivum L.,cv.Yangmai 13） seedlings to heavy metal（Cd,Hg） and 1,2,4-trichlorobenzene（TCB） stresses were examined by two-dimensional gel electrophoresis,image analysis,and peptide mass fingerprinting.The results showed inhibitions of root and shoot growth by Cd,Hg,and TCB.These stresses led to water deficit and lipid phosphorylation in the seedling which also promoted protein phophorylation in the leaves.Hg stress inhibited protein synthesis while Cd and TCB stresses induced or up-regulated more proteins in the leaves.Most of these induced proteins played important roles in the biochemical reactions involved in tolerance of wheat to Cd and TCB stresses.The primary functions of Cd-and TCB-induced proteins included methionine metabolism,Rubisco modification,protein phosphorylation regulation,protein configuration protection,H＋ transmembrane transportation and also the synthesis of ethylene,defense substances and cell wall compounds.

关 键 词：WHEAT PROTEOMICS chemical pollutant stress response 

分 类 号：S512.101[农业科学—作物学] TQ242.1[化学工程—有机化工]