β-环糊精/芳环氨基酸包络物的电喷雾质谱研究  

Studies on β Cyclodextrin amino Acids Inclusion Complexes by Electrospray Ionization Mass Spectrometry

作　　者：孙维星[1] 刘志强[1] 宋凤瑞[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所

出　　处：《高等学校化学学报》1998年第5期708-710,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：近年来，电喷雾质谱作为“软电离”质谱新技术广泛应用于生命科学领域［１，２］，１９９１年Ｇａｎｅｍ等［３］首次利用电喷雾质谱（ＥＳＩ－ＭＳ）对溶液中特异性免疫蛋白质－药物复合物进行了研究．目前生物酶－底物／抑制剂、ＤＮＡ－药物、蛋白质－配体及生物大分子...The positive and negative ion electrospray ionization mass spectra of β cyclodextrin amino acids complexes in NH 4Ac buffer have been reported in this paper. Compared with positive ion ESI mass spectra of β cyclodextrin amino acids complexes under the same condition, negative ion mass spectra obtained for inclusion complexes of β cyclodextrin (CD) with tyrosine, phenylalanine and tryptophan, respectively, were completely dominated by deprotonated complex ions and CD-H - ion which is the only daughter ion in collision induced dissociation (CID) experiment of deprotonated complexes. The results indicated that the charged position for protonated and deprotonated complexes is different from each other. In addition, two complex ions for the same complex have similarly relative dissociation energies, which are higher than that of CD+NH 4 +, indicating that complexes observed in gas phase are not electrostatic adducts at all but complexes formed by hydrogen bonds.

关 键 词：Β-环糊精 包络物 电喷雾质谱 环糊精 ESIMS 

分 类 号：O636.12[理学—高分子化学]