海南黑山羊生长激素(GH)基因单核苷酸多态性分析  

Single Nucleotide Polymorphism Analysis of Hormone (GH) Growth Gene in Hainan Black Goat

作　　者：周汉林[1] 管松[1] 李琼[1] 王东劲[1] 侯冠彧[1] 曾鸿普[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《热带农业科学》2009年第4期31-35,共5页Chinese Journal of Tropical Agriculture

基　　金：海南省自然科学基金(No.80304)资助

摘　　要：利用PCR-SSCP与测序相结合,分析海南黑山羊GH基因第2外显子exon2的多态性,SSCP结果出现3种基因型,分别定义为AA、AB、BB;3种基因型频率分别为28.3%、63.0%、8.7%,A、B等位基因频率分别为59.8%和40.2%,以A等位基因为优势基因,经Hardy-Weinberg平衡卡方适合性检验结果表明,exon2在此群体中处于Hardy-Weinberg不平衡状态(X2=0.000,P<0.01),该位点群体遗传结构及多态指标分析结果显示,该位点表现为中度多态。AA和BB基因型进行测序结果发现,exon2第11位点发生G→C突变,将核苷酸序列翻译成氨基酸序列进行同源性比较,结果exon2第11位G→C突变位点引起编码的氨基酸改变,所编码的氨基酸由原来的精氨酸变为脯氨酸。The polymorphism of Hainan black goat GH gene exon 2 was analysed by PCR-SSCP and sequencing. The SSCP result showed that 3 genotypes were discovered and defined as AA, AB, BB with their frequencies being 28.3%, 63.0%, 8.7% , respectively, and the A, B allele frequencies being 59.8% and 40.2%. The Hardy-Weinberg balance for Chi-square test with allele gene of B as the dominance shows that the exon2 was imbalanced in the group at the Hardy-Weinberg （X2=0.000, P〈0.01）. Population genetic structure and polymorphism analysis showed that the site of the GH gene exon2 was moderate. The AA and BB genotype sequencing showed that the 1 lth point of GH gene exon2 was mutated （G→C）, which was then translated into the sequence of amino acid sequence for homology comparison. The homology comparison showed that the mutation caused the change of the coded amino acid which is changed from arginine to proline.

关 键 词：海南黑山羊 GH基因 单核苷酸多态性 氨基酸 

分 类 号：S827[农业科学—畜牧学] Q524.3[农业科学—畜牧兽医]