用十二烷基磺酸钠纯化重组大肠杆菌中的聚-β-羟基丁酸酯  

RECOVERING POLY-β-HYDROXYBUTYRATE FROM RECOMBINAN T E.coli BY SODIUM-DODECYL SULPHATE

作　　者：尹进[1] 于慧敏[1] 李红旗[1] 沈忠耀[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《化工学报》1998年第3期353-357,共5页CIESC Journal

摘　　要：A method for recovering PHB（poly- -hydroxybutryate）from recombinant E.coli by using surfactant SDS（sodium-dodecyl sulphate） was studied.The factors,including reaction time, temperature,concentration of SDS and pH were optimized.The results showed that temperature had no effect on the purity of PHB,while SDS treated biomass for more than 10 minutes at a higher pH or concentration of SDS could result in higher PHB with lower molecular weight.At 20℃ and pH12,when 3g·L-1 SDS was used to treat 30g·L-1 cells for l0 minutes,83.05% proteins in the cells could be removed,whereas the purity of PHB was increased from 71.86% to 95.18% and the molecular weight of PHB was 983000（the initial molecular weight was 1180000）.A method for recovering PHB(poly- -hydroxybutryate)from recombinant E.coli by using surfactant SDS(sodium-dodecyl sulphate) was studied.The factors,including reaction time, temperature,concentration of SDS and pH were optimized.The results showed that temperature had no effect on the purity of PHB,while SDS treated biomass for more than 10 minutes at a higher pH or concentration of SDS could result in higher PHB with lower molecular weight.At 20℃ and pH12,when 3g·L^(-1) SDS was used to treat 30g·L^(-1) cells for l0 minutes,83.05% proteins in the cells could be removed,whereas the purity of PHB was increased from 71.86% to 95.18% and the molecular weight of PHB was 983000(the initial molecular weight was 1180000).

关 键 词：聚羟基丁酸酯 PHB 分离 SDS 纯化 大肠杆菌 

分 类 号：TQ929[轻工技术与工程—发酵工程] TQ323.41