大鼠PDLLA/NGF套管模型与自体神经移植模型脊髓背角c-fos表达的比较研究  

Comparative Study on c-fos Expression in Cornu Dorsale Medullae Spinalis of Rat between Nerve Autograft Model and PDLLA/NGF Compound Condit Model

作　　者：邬明[1] 唐忠志[1] 陈谦[1] 程青[1] 

机构地区：[1]解放军广州军区武汉总医院急诊科,湖北武汉430070

出　　处：《临床军医杂志》2009年第3期346-349,共4页Clinical Journal of Medical Officers

基　　金：湖北省卫生厅科研基金资助项目(JX2B45)

摘　　要：目的建立大鼠PDLLA/NGF(外消旋聚乳酸/神经生长因子)复合套管模型和大鼠外周神经自体神经移植神经再生模型,观察对比各模型神经恢复过程中机械痛觉超敏的变化及脊髓背角c-fos表达的改变,探讨两种神经再生模型中神经再生与神经性疼痛的联系。方法雄性Wistar大鼠60只随机分成3组,A组为自体神经移植再生模型组;B组为PDLLA/NGF套管修复模型组;C组假手术组。分别制成模型后,术后18d起检测机械刺激阈值及c-fos表达计数。所有数据采用均值±标准差(x±s)表示,组间采用t检验,用SPSS11.0软件进行统计分析,P<0.05为有统计学差异。结果A、B组各项指标与C组比较有显著差异;A组与B组比较c-Fos表达阳性细胞计数及机械痛阈无显著差异。结论两种模型再生神经生长进入去神经支配区域时,均出现支配区域的痛觉超敏和疼痛相关行为,神经性疼痛程度无差异。Objective To observe mechnic allodynia and c-fos expression changes between PDLLA/NGF compound condit model and nerve autograft model after models of rat were built, and study the corelation between two nerve regeneration model and neure-pathic pain. Methods 60 male wistar rats were randomly divided into four groups ： sciatic nerve autograft （group A, n = 20） PDL- LA/NGF compound condit group （ group B, n = 20 ） and sham-operation（group C, n = 20）. After the model were built, on the 18d, we tested sensitivity threshold and c-fos counting. All data were described as mean and standard deviation（^-x±s） , tested by means of T test, and statistically analyzed by SPSS11.0 software. P 〈0.05 is set as having statistical significance. Results Compared with group C, all indexes in group A and B have significant difference; Compared with group B, sensitivity threshold and c-fos counting in group A have no significant difference. Conclusion When the injured fibers have regrown into the nerve-yield region, both two model of nerve regeneration produced allodynia and pain-related behavior. There is no significant difference in the degree of neuropathic pain.

关 键 词：周围神经损伤 神经再生 神经性疼痛 痛觉过敏 c-fos PDLLA/NGF复合套管 

分 类 号：R745[医药卫生—神经病学与精神病学]