气相色谱/质谱法测定大鼠脑中5-羟色胺的含量  

Determination of Serotonin in Rat Brain by Gas Chromatography/Mass Spectrometry

作　　者：梁天天 黄亦佳[1,2] 朱卡琳[1,2] 

机构地区：[1]浙江省药学会 [2]浙江医科大学药学院

出　　处：《色谱》1998年第3期271-273,共3页Chinese Journal of Chromatography

摘　　要：采用气相色谱／质谱法（ＧＣ／ＭＳ）测定了正常大鼠和服药大鼠脑组织中５－羟色胺（５－ＨＴ）的浓度。大鼠脑组织制成匀浆后，先将５－ＨＴ酰化，酰化物经乙酸乙酯提取后，再经七氟丁酸酐衍生化，用ＧＣ／ＭＳ测定。ＭＳ采用电子捕获负离子化学电离（ＥＣＮＩＣＩ）模式。方法的线性范围为０．５０～５０．０μｇ／Ｌ，回归方程为Ｙ＝０．１３４８Ｘ－０．０７９９５（ｒ＝０．９９９６）；平均回收率为９８．２％±３．８％（ｎ＝１０）；检测限为０．５μｇ／Ｌ；相对标准偏差小于１０％。A GC/MS method for the determination of serotonin (5 hydroxytryptamine, 5 HT) has been developed. 5 HT in homogenized rat brain was acylated and extracted into EtOAc. The acylate was further derivatized with heptafluorbutyl anhydride and formed a spirocyclic pentafluoropropionyl derivative. Subsequent analysis of 5 HT derivative by gas chromatography electron capture negative ion chemical ionization mass spectrometry was highly specific and sensitive. The assay is linear from 0.5 to 50μg/L with a linear equation of Y=0.134 8X-0.079 95,r=0.999. The recovery was 98.2%±3.8% ( n=10) and the limit of detection was 0.5μg/L. The reproducibility of 5 HT determination in rat brain was less than 10% (RSD) at both levels of 1.0μg/L and 50.0μg/L. The method has been applied to analysis of 5 HT in the dose rat brain successfully.

关 键 词：GC MS 5-羟色胺 大鼠脑组织 羟色胺 测定 分析 

分 类 号：Q422[生物学—神经生物学]