人心果原生质体分离初探  

Preliminary Study on the Protoplasts Isolation of Manilkara zapota

作　　者：罗丽华[1] 杨定海[1] 林尤河[1] 王业华[1] 

机构地区：[1]海南大学园艺园林学院,海南海口570228

出　　处：《福建林业科技》2009年第2期127-130,共4页Journal of Fujian Forestry Science and Technology

摘　　要：以人心果幼嫩叶片组织为材料,研究了酶液组合、酶解时间、酶液中甘露醇含量、pH值、温度等因素对其原生质体分离的影响,结果表明:以3%纤维素酶R-10+0.4%果胶酶Y-23为混合酶液,0.6 mol.L-1甘露醇为渗透压稳定剂,pH 5.6的酶液组合,在28℃的黑暗条件下振荡,酶解时间2 h,分离出的原生质体产量最佳,可达到12.4×106个.g-1。The tender leaves of Manilkara zapota were used as experiment materials and the influences of enzyme solution combinations, zymohydrolysis time and mannitol concentration in the enzyme solution , pH value, and temperature on protoplast isolation were studied. The results showed that the highest yield （12.4 × 10^6/g） was obtained by treating the leaves with a mixture of 3 % of cellulose enzyme R-10 ＋ 0.4 % of pectase Y-23 with pH value of 5.6 for 2h of zymohydrolysis time at the temperature of 28℃, and on the oscillation condition with 0.6 mol. L^-1 of mannitol as the osmotic pressure stabilizer.

关 键 词：人心果 原生质体分离 原生质体产量 

分 类 号：S667.9[农业科学—果树学]