压力对采油微生物生长代谢的影响  

Effects of pressure on growth and metabolism of microorganism for microbial enhanced oil recovery

作　　者：王登庆[1,2] 郭辽原[2] 刘涛[3] 段传慧[2] 张君[2] 宋智勇[2] 

机构地区：[1]中国石油大学石油工程学院,山东东营257061 [2]中石化胜利油田采油工艺研究院,山东东营257000 [3]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100

出　　处：《中国石油大学学报（自然科学版）》2009年第3期114-117,127,共5页Journal of China University of Petroleum(Edition of Natural Science)

基　　金：'十一五'国家科技支撑计划项目(2006BAB04B02);中石化科技攻关项目(P05076)

摘　　要：影响微生物提高原油采收率技术的油藏环境因素主要包括压力、温度、矿化度和pH值等。以葡萄糖为主要碳源,对河口采油厂沾3x24井产出水中的内源微生物进行高压(10 MPa)和常压下的静态激活试验,对培养前后的微生物群落结构、代谢产生的低分子有机酸及表面张力进行检测。结果表明:微生物在不同压力下的生长代谢有较大的差异,压力不仅显著影响微生物群落的结构,而且影响微生物的生长代谢活性和速度,以及整体群落的代谢方式;与高压相比,常压下微生物种类较多,丰度较高,代谢高峰提早14 d,变化周期较短。The major environmental factors affecting microbial enhanced oil recovery （MEOR） are pressure, temperature, salinity, pH, and so on, By using glucose as major carbon source, the in-situ microorganism of produced water from Zhan 3×24, Hekou Oil Plant was cultured under high pressure （ 10 MPa） and atmospheric pressure. Meanwhile, the community structures, low molecular organic acid and surface tension were analyzed both before and after the culture. The results show that pressure affects community structures, growth and metabolic activities or pathways. Compared with high pressure, there were many species microorganism in abundance under atmospheric pressure, and the metabolic summits appeared 14 days early with short periods.

关 键 词：油藏压力 采油微生物 生长代谢 群落结构 提高采收率 

分 类 号：TE355.9[石油与天然气工程—油气田开发工程]