PRRSV JL/07/SW毒株GP5重组蛋白间接ELISA检测方法的建立  

Establishment of GP5-ELISA Diagnosis Method for JL/07/SW Strain of Reproductive and Respiratory Syndrome Virus

作　　者：高恩鹏[1] 丁壮[2] 高原[1] 孟轲音[3] 宣华[4] 王贵平[4] 

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]军事医学科学院军事兽医研究所,吉林长春130062 [4]广东省农科院兽医研究所,广东广州510000

出　　处：《吉林工程技术师范学院学报》2009年第4期72-74,共3页Journal of Jilin Engineering Normal University

摘　　要：本研究将以纯化的重组GP5蛋白作为包被抗原,建立了诊断PRRS的间接ELISA方法。实验确定了最佳抗原包被浓度为2.30μ/mL,PRRSV阳性血清稀释度为1∶100。用间接ELISA方法检测猪瘟、猪细小病毒、猪戊肝病毒阳性血清无交叉反应,证明有很好的特异性。Using recombined fusion GP5 protein as coating antigen set up a method of indirect enzyme - linked immtmosorbent assay （ELISA） to diagnose PRRSV. The experiment made sure of the optimal primordial covering concentration was 2.30 μg/mL. The optimal dilution of serum examinod was 1： 100. To check out among PPI（poreine parvovims infection） ; CSF（Classical swine fever ）and HEV（hybrid electric vehicle） positive serum, which weren＇t have no cross reaction by indirect ELISA method, it improved that this method had strong specificity.

关 键 词：重组质粒 重组GP5蛋白 间接ELISA 

分 类 号：S854.4[农业科学—临床兽医学]