二次膜分离提取藏牦牛血凝血酶的工艺  

Crafts to extract thrombin from the blood of the Tibet yak by double-membrane separation

作　　者：曹芳 李耀曾[2] 杜兴[2] 夏华[2] 

机构地区：[1]平凉市药品检验所,甘肃平凉744000 [2]甘肃省药品检验所,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2009年第2期51-53,共3页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的探讨二次膜分离提取藏牦牛血凝血酶的工艺。方法根据藏牦牛血凝血酶原及其凝血酶的分子量,进行二次膜分离技术提取藏牦牛血凝血酶。结果采用二次膜分离技术提取藏牦牛血凝血酶,其比活力与活力回收分别比有机溶剂沉淀法提高了76.72 U/mg蛋白质和35.0%;其比活力比一次膜分离法提高了41.14 U/mg蛋白质。结论工艺可靠,方法可行,适宜于中小企业的生产。Objective To establish crafts to extract thrombin from the blood of the Tibet yak by double-membrane separation. Methods According to molecular weights of blood thrombinogen and thrombin of Tibetan yak, thrombin from the blood of the Tibet yak was extracted by double-membrane separation. Results The specific activity and activity recollection of thrombin were improved by 76.72 U/mgpro and 35.0% respectively when compared with organic solvent precipitation method, and the specific activity of thrombin was improved by 41.14 U/mg pro when compared with single membrane separation. Conclusion The craft is credible and feasible, the and can be used for the production of small and medium-sized scales.

关 键 词：二次膜分离 藏牦牛血 凝血酶 凝血酶原 

分 类 号：R931.73[医药卫生—生药学]