甲基叔丁基醚(MTBE)降解菌株的分离鉴定及降解动力学研究  

Isolation,Identification of Methyl Tert-Butyl Ether Degradation Strain and Its Degradation Kinetics

作　　者：张杏青[1] 朱妙军[1] 胡勤海[1] 王丹[1] 武丽丽[1] 

机构地区：[1]浙江大学环境工程系,杭州310029

出　　处：《环境科学》2009年第6期1785-1790,共6页Environmental Science

摘　　要：从银杏根部土壤中分离驯化出1株能以MTBE为唯一碳源生长的菌株A1,经过16S rDNA序列分析后,初步鉴定该菌株为睾酮丛毛单胞菌(Comamonas testosterone);进一步试验研究了微生物接种量、pH值、温度、MTBE初始浓度等环境因素对MTBE降解的影响.结果表明,菌A1降解MTBE的最佳条件为:pH值7.0,温度25℃,接种量2 mL(D600=2.523 A),初始MTBE浓度50mg/L.在此降解条件下,7 d后MTBE去除率最高可达98.89%(与空白实验对比,MTBE挥发量占46.55%).菌A1降解MTBE符合高浓度底物抑制的酶促反应类型,其基本降解动力学参数rmax=0.872 d-1,Km=7.832 mg.L-1,Ks=130.75 mg.L-1.A methyl tert-butyl ether degradation strain Al was isolated from the soil under an old Gingko tree. It was identified preliminarily as Comamonas testosterone by 16S rDNA sequence analysis. The main factors including inoculation amount of microbes, pH, temperature and MTBE concentration that may affect the degradation efficiency of MTBE were further studied. The results indicated that the optimum conditions were as following： pH 7.0, temperature 25℃, inoculation amount of microbes was 2 mL（ D600 = 2.523 A）, initial MTBE concentration 50 mg/L. Under that condition, MTBE can be reduced by 98.89 % with seven days （compared with the blank, the volatilization of MTBE was 46.55 % ）. In addition, the biodegradation process of MTBE can be well described by enzymatic reaction of high concentration inhibition, with the maximum substrate utilization rate 0.872 d^-1, Michaelis-Menten constant 7.832 mg·L^-1, inhibitory constant 130.75 mg·L^-1 respectively.

关 键 词：MTBE 生物降解 睾酮丛毛单胞菌(Comamonastestosterone) 降解动力学 

分 类 号：X172[环境科学与工程—环境科学]