非灭菌条件下酸性蓝45在白腐真菌反应器中的降解特性  

Degradation of Acid Blue 45 in a White-Rot Fungi Reactor Operated Under(Nonsterile) Conditions

作　　者：周成[1] 文湘华[1] 

机构地区：[1]清华大学环境科学与工程系,环境模拟与污染控制国家重点实验室,北京100084

出　　处：《环境科学》2009年第6期1797-1801,共5页Environmental Science

基　　金：国家自然科学基金项目(20677033)

摘　　要：在非灭菌条件下维持白腐真菌反应器连续运行是在水处理领域实际应用白腐真菌的前提.采用臭氧控制白腐真菌反应器中的污染细菌,以连续运行方式考察了自由悬浮培养的白腐真菌体系中控菌、产酶、pH变化及对酸性蓝45的降解特性.结果表明,在白腐真菌反应器系统的控菌单元,以0.0144 mg/min臭氧投加速率,可成功将白腐真菌反应器内污染细菌控制在1×10^6CFU/mL以下,其去除率接近99%;成功实现了白腐真菌反应器连续合成锰过氧化物酶（最大剩余酶活50 U/L）和对酸性蓝45的持续降解（40%-80%）;发现pH值在6左右时,白腐真菌培养体系仍然具有合成锰过氧化物酶和对酸性蓝45的持续降解的能力;提出在控制污染细菌的前提下,如何维持白腐真菌的持续生长及高产酶是进一步实现白腐真菌反应器连续运行的主要问题.To operate the white-rot fungal reactor under non-sterile conditions has important significance for its practical applications. Ozone （0.0144 mg/min）was selected as the bactericide to control the bacterial contamination in a white-rot fungal reactor. The control effect on bacteria, enzyme production, pH variation and decolorization of a recalcitrant dye （ acid blue 45 ） in the reactor were studied during continuously operating the reactor. It was found that, the contamination bacteria were controlled at under a number of 1 ×10^5-1×10^6 CFU/mL and the inactivation rate of contaminating bacteria in white-rot fungal reactor was close to 99%. The Phanerochaete chrysosporium continuously produced MnP with the maximum residual activity of 50 U/L and degraded the acid blue 45 with a rate of 40%-80%. At around pH 6, the system still had the capability to continuously produce MnP and degrade the acid blue 45. To keep the growth of white-rot fungi and production of MnP for a long term operation is a next step point to be solved.

关 键 词：白腐真菌反应器 酸性蓝45 PHANEROCHAETE CHRYSOSPORIUM 臭氧 细菌污染 锰过氧化物酶 

分 类 号：X172[环境科学与工程—环境科学] X505