家猪13/17罗伯逊易位染色体着丝粒DNA的FISH检测  

Detection of 13/17 Robertsonian Translocation Chromosome Centromeric DNA by FISH in the Domestic Pig

作　　者：何晓波[1] 侯永刚[1] 孙泰雷[1] 柏蒙蒙[1] 孙金海[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109

出　　处：《青岛农业大学学报（自然科学版）》2009年第2期90-94,共5页Journal of Qingdao Agricultural University(Natural Science)

基　　金：国家自然科学基金资助项目(30871778);教育部科学技术研究重点项目(J06007)

摘　　要：根据已报道的家猪着丝粒卫星DNA序列设计引物,对家猪基因组DNA进行PCR扩增,得到AC2卫星DNA序列。用PCR法将D ig-dUTP插入目的片段中得到标记DNA,然后利用荧光原位杂交方法检测13/17易位染色体。研究结果显示杂交信号位于所有端着丝粒染色体和13/17易位染色体的着丝粒区域,表明13号染色体和17号染色体虽然融合为一条亚中着丝粒染色体,但仍含有端着丝粒AC2卫星DNA,13/17易位染色体的AC2卫星DNA未发生缺失。Primers were designed to amplify AC2 satellite DNA of pigs based on the centromere satellite DNA sequences, and the amplification products were labeled with DigdUTP by PCR to detect the 13/17 translocation chromosomes by FISH. The results showed that the hybridization signals were located in the centromeric regions of all acrocentric chromosomes and 13/17 translocation chromosomes. It could be concluded that the acrocentric AC2 satellite DNA was still in the centromeric regions of 13/17 Robertsonian translocation chromosomes although the 13 chromosome and 17 chromosome would fuse into subcentromeric chromosomes. Also, no deletion of AC2 satellite DNA of 13/17 translocation chromosomes was found in detected pigs.

关 键 词：家猪 AC2家族 罗伯逊易位 荧光原位杂交 

分 类 号：Q953.3[生物学—动物学]