见血封喉内生真菌的分离鉴定及抑菌活性研究  

Isolation,identification,and antimicrobial activity of endophytic fungi from Antiaris toxicaria

作　　者：戴文君[1] 张秀环[1] 黄贵修[2] 戴好富[1] 梅文莉[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《中草药》2009年第6期955-957,共3页Chinese Traditional and Herbal Drugs

基　　金：973计划前期研究专项(2007CB116306);海南省自然科学基金资助项目(30608);海南省自然科学基金指导性项目(80671)

摘　　要：目的分离和鉴定见血封喉Antiaris toxicaria根韧皮部中的内生真菌,并检测其抑菌活性。方法以金黄色葡萄球菌Staphylococcus aureus、耐甲氧西林金黄色葡萄球菌methicillin-resistantStaphylococcus aureus(MRSA)和白色念珠菌Candida albicans作为指示菌,采用杯碟法对内生真菌发酵上清液进行抑菌试验。结果从见血封喉根的韧皮部中分离出6株内生真菌,经形态鉴定分属于聚端孢霉属(Trichothecium LK.ex Fr.)、枝顶孢霉属(Acremonium LK.ex Fr.)和丝核菌属(Rhizoctonia DC.ex Fr.)。其中内生真菌J6的发酵上清液对MR-SA有抑菌活性,抑菌圈直径为18 mm。结论首次对见血封喉内生真菌进行了研究,内生真菌J6对MRSA有明显的抑菌活性,其抑菌活性成分值得进一步研究。Objective To isolate and identify endophytic fungi from the phloem in the root of Antiaris toxicaria and detect the antimicrobial activity of these strains. Methods Staphylococcus aureus, methicillin resistant Staphylococcus aureus （MRSA）, and Candida albicans were selected as indicators. The anti- microbial activities of the fermentation filtrate of endophytic fungi were tested by cylinder plate method. Results Six endophytic fungi were isolated from the phloem in the root of A. toxicaria. These strains were morphologically identified to genus of Trichotheciurn I.K. ex Fr. , Acremonium LK. ex Fr. and Rhizoctonia DC. ex Fr. The fermentation filtrate of endophytic fungus J6 showed the antimicrobial activity against MRSA. The diameter of inhibition zone was 18 mm. Conclusion It＇s the first report about the endophytic fungi from A. toxicaria. Endophytic fungus J6 exhibits strong antimicrobial activity against MR- SA, its active components are of worth to further study.

关 键 词：见血封喉 内生真菌 抑菌活性 

分 类 号：R282.2[医药卫生—中药学]