茶树PCP基因内含子的克隆与序列分析  

Cloning and analyzing of the intron of Tea PCP gene

作　　者：陈聪[1] 江昌俊[1] 叶爱华[1] 谭振[1] 童鑫[1] 

机构地区：[1]安徽农业大学农业部茶叶生物化学与生物技术重点开放实验室,安徽合肥230036

出　　处：《安徽农学通报》2009年第11期69-69,190,共2页Anhui Agricultural Science Bulletin

摘　　要：利用GenBank中公布的茶树花粉壁蛋白基因(PCP)序列设计引物,采用PCR法扩增并测定茶树花粉壁蛋白基因内含子序列。在该基因中发现一个内含子,共207bp,A+T碱基占67.63%,G+C碱基占32.37%。经分析发现该内含子具有类似启动子的序列存在,可能对茶树花粉壁蛋白的特异表达有调控作用。According to the pollen coat protein gene （PCP） of tea in GenBank, the primer were designed and the intron of Tea PCP gene was cloned using polymerase chain reaction （PCR） technique, a 207 base pairs was obtained, the content of A ＋ T, G ＋ C was 67. 63% ,32. 37%. The special structure in the introns may indicate the role that they may play in pollen coat protein gene expression. The analogous structure of promoter in the introns may indicate the role that they may play in pollen coat protein gene expression.

关 键 词：内含子 序列 分析 花粉壁蛋白 茶树 

分 类 号：Q78[生物学—分子生物学] O652.7[理学—分析化学]