线栓法制备大鼠脑缺血再灌注模型的方法研究  

Establishment a Model of Rat Ischemia-reperfusion Injury with Intraluminal Suture

作　　者：马贤德[1] 孙宏伟[1] 柴纪严[1] 赵金茹[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《中华中医药学刊》2009年第6期1200-1201,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512707)

摘　　要：目的:建立一种比较系统,操作简单,成功率高的大鼠大脑中动脉缺血(MACO)再灌注动物模型,达到只要读者根据本文所述的方法操作就能制作出MACO再灌注模型的目的。方法:成年健康雄性SD大鼠40只,参照Longa法并适当改进建立MACO模型20只,假手术组20只。将详细叙述手术过程以及再灌注时间点的合理选择。最后利用行为学测试、四氮唑(TTC)染色对模型成功与否进行判定。结论:线栓法是一种操作简单的制备MACO再灌注动物模型的方法,并且此方法的再灌注效果较为明显。Objective： To establish a model of rat isehemia - reperfusion injury, in terms of the model, the operation will be simple,and the achievement ratio will be high. Methods：40 Male Sprague-Dawley（SD）rats were separated into two groups. Results：20 were model of rat ischemia-reperfusion injury based on Longa method,and the other 20 were sham-operated group. The process of the operation and the selection of different time point following isehemie-reperfusion were diseussed in the paper. What＇s more, the model was appraised by behavioral test and Triphenyl Tetrazolium Choloride（TTC） Staining. Conclusion ：The operation of intraluminal suture method is very simple for the establishment of model of rat ischemia-reperfusion,what＇s more ,the effect of reperfusion is very obvious.

关 键 词：动物模型 脑缺血 再灌注 线栓法 

分 类 号：R-33[医药卫生]