医院感染病原菌AmpC与超广谱β-内酰胺酶检测  

Detecting and Analyzing AmpC β-Lactamase and ESBLs in Gram-negative Bacilli from Nosocomial Infection

作　　者：王寅[1] 赵虎[2] 孟丽[1] 夏华峰[1] 尤佳女[1] 

机构地区：[1]浙江中医药大学附属第二医院检验科,浙江杭州310005 [2]复旦大学附属华东医院检验科,上海200040

出　　处：《中华医院感染学杂志》2009年第11期1425-1427,共3页Chinese Journal of Nosocomiology

基　　金：浙江省医药卫生科研基金资助项目(2006A090)

摘　　要：目的了解医院感染病原菌中革兰阴性杆菌产AmpC与超广谱β-内酰胺酶(ESBLs)的情况。方法用改良的头孢西丁三维试验和头孢曲松三维试验检测持续高产AmpCβ-内酰胺酶和ESBLs。结果医院感染病原菌中革兰阴性杆菌持续高产AmpCβ-内酰胺酶,产酶率为16.00%,其中单独产AmpCβ-内酰胺酶的产酶率为8.84%,以阴沟肠杆菌、褪色沙雷菌和产气肠杆菌产酶率高(36.00%、31.25%和28.00%);同时产ESBLs的检出率为7.16%,以鲍氏不动杆菌、肺炎克雷伯菌和大肠埃希菌阳性率高(15.18%、10.60%和8.74%)。结论医院感染菌株产酶率较高,应引起临床高度重视。OBJECTIVE To investigate the productive rate of AmpC β-laetamases and ESBLs produced by the Gramnegative bacilli from the nosoeomial infection in our hospital. METHODS AmpC β-lactamases and ESBLs were detected by the improved eefotaxime and ceftriaxone three-dimensional test. RESULTS The productive rate of AmpC β-laetamases was 16.00%. Among them, the productive rate of AmpC β-lactamases was only 8.84%. The Enterobacter cloacae, Serratia marcescens and Enterobacter aerogenes were easy to produce the enzymes （36.00%, 31.25% and 28.00%）. The productive rate of AmpC β-laetamases and ESBLs at the same time was 7.16%. CONCLUSIONS The productive rate of the enzymes by the Gram-negative bacilli from the nosocomial infection is rather high.

关 键 词：AMPC Β-内酰胺酶 超广谱Β-内酰胺酶 医院感染 三维试验 

分 类 号：R378[医药卫生—病原生物学]