丹参酮ⅡA诱导人喉癌细胞Hep-2凋亡的研究  

Study on Apoptosis of Laryngeal carcinoma Hep-2 Induced by Tanshinone ⅡA

作　　者：卢岩[1] 康健[1] 

机构地区：[1]辽宁医学院附属第一医院耳鼻咽喉-头颈外科,辽宁锦州121001

出　　处：《辽宁中医杂志》2009年第6期880-881,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:研究丹参酮ⅡA对人喉癌细胞Hep-2的生长抑制作用及凋亡诱导作用。方法:四亚基噻唑蓝(MTT)法测定丹参酮ⅡA对细胞生长的抑制作用,流式细胞仪技术(FCM)观察细胞周期及凋亡。结果:不同浓度的丹参酮ⅡA均能抑制人喉癌细胞Hep-2生长,并有明显的时间和剂量依赖性;流式细胞仪检测不同浓度丹参酮ⅡA作用48h后的细胞凋亡率增高,与对照组比较差异均有统计学意义。结论:丹参酮ⅡA在体外能明显抑制人喉癌细胞Hep-2生长,抑制其生长的机制可能是诱导细胞凋亡。Objective ：To determine the effect of tanshinone Ⅱ A on the growth and apoptosis in human Laryngeal carcinoma Hep - 2 cells. Methods： Using MTT assay, the growth inhibiting rate of tanshinone Ⅱ A on Hep - 2 cells was dedected. Flow cytometry（FCM） were applied to demonstrate the presence of apoptosis and cell cycle. The expression of Bax and bcl -2 was analyzed with Western blot. Results：Tanshinone Ⅱ A inhibited the growth of Hep -2 cells in a time - and dose -dependent manner. The apoptotic rates were increased, which were all significantly higher than those of the control group. Conclusion ： Tanshinone Ⅱ A can inhibit the proliferation of human Laryngeal carcinoma Hep - 2 cells in a time - and dose - dependent manner and the mechanism of growth inhibition of human Laryngeal carcinoma ceUs may be related to the induction of apoptosis.

关 键 词：丹参酮ⅡA 喉癌细胞 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]