栽培曼地亚红豆杉针叶化学成分的分离与鉴定  

Isolation and identification of chemical constituents from the needles of Taxus media

作　　者：张娜[1] 路金才[1] 王晶[1] 韩凌 杨晓光[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]国家中成药工程技术研究中心,辽宁本溪117004

出　　处：《沈阳药科大学学报》2009年第6期434-437,472,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的对栽培于我国辽宁本溪的曼地亚红豆杉(Taxus media)针叶的化学成分进行研究。方法采用大孔树脂、硅胶柱色谱、ODS柱色谱、凝胶柱色谱及MDS柱色谱等方法进行分离纯化,通过NMR数据分析鉴定化学结构。结果从曼地亚红豆杉针叶的乙醇提取物中分离得到8个化合物,分别鉴定为二萜类化合物:紫杉宁(taxinine,1)、东北红豆杉素(taxacin,2)、紫杉欧吉酚(taxa-gifine,3)、2-去乙酰氧基紫杉宁J(2-deacetoxytzxinine J,4)、东北红豆杉素I5-O-桂皮酰基三乙酸酯(5-O-cinnamoyltaxacin I triacetate,5);黄酮类化合物:芹菜素(apigenin,6)、银杏双黄酮(ginkget-in,7)、金松双黄酮(sciadopitysin,8)。结论化合物2、5、7、8为首次从栽培曼地亚红豆杉针叶中分离得到。Objective To investigate chemical constituents from the needles of Taxus media growing in Liaoning Province of China. Methods The needles of the plant were extracted with ethanol, the ethanol extract was submitted to chromatography on silica gel column, ODS, MDS and Sephadex LH-20 to isolate some compounds. Their structures were elucidated on the basis of spectral analysis. Results Eight compounds were isolated and identified as taxinine （ 1 ）, taxacin （2）, taxagifine （3）, 2-deacetoxytaxinine J （4）, 5-O-cinnamoyl- taxacin I triacetate （ 5 ）, apigenin （6）, ginketin （ 7 ）, sciadopitysin （ 8 ）. Conclusions 2,5,7 and 8 are isolated from the needles of Taxus media for the first time.

关 键 词：曼地亚红豆杉 紫杉烷二萜 黄酮类化合物 化学成分 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R284[医药卫生—药学]