PRRSVJL/07/SW毒株GP5基因克隆与原核表达  

Cloning and expression of GP5 gene JL/07/SW strain of porcine reproductive and respiratory syndrome virus

作　　者：高恩鹏[1,2] 李志杰[2] 丁壮[2] 孟轲音[3] 宣华 王贵平 丛彦龙[2] 母连志[2] 

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]军事医学科学院军事兽医研究所,吉林长春130062 [4]广东省兽医研究所,广东广州510000

出　　处：《中国兽医学报》2009年第6期688-690,695,共4页Chinese Journal of Veterinary Science

基　　金：广东省科技攻关重大项目(2008A0201000020)

摘　　要：根据GenBank发表的猪繁殖与呼吸综合征病毒(PRRSV)VR-2332毒株核苷酸序列,设计了扩增PRRSV JL/07/SW毒株GP5基因的1对引物,利用RT-PCR方法,从PRRSV JL/07/SW毒株的细胞培养物中成功的扩增出GP5基因,其中GP5基因全长603 bp,与国际PRRSV美洲型VR-2332毒株的GP5基因序列同源率为89.2%;随后,将该基因与原核表达载体pGEX-4T-1连接,转化到宿主菌BL21中进行表达。结果证实PRRSV JL/07/SW毒株GP5基因成功地进行了原核表达,表达的目的蛋白占菌体总蛋白含量的22.8%。According to the PRRSV VR-2332 strain nucleotide sequence reported in the GenBank. One pair of primer was designed to amplify PRRSV JL/07/SW strain GP5 gene. The GP5 gene of PRRSV JL/07/SW strain has been successfully amplified by RT-PCR from the cell cultures of PRRSV JL/07/SW. The whole GP5 gene has 603 basic in length,with 89.20/00 gene order homogeneity to the PRRSV Europe-type VR 2332 strain;The GP5 gene has been ligated with pGEX-47-1 and transformed into BL21. The result shows that the GP5 gene of PRRSV JL/07/SW strain has been well carried out to prokaryotic expression. The content of the target protein reaches 22.8% in the whole proteins of the host strains.

关 键 词：PRRSV GP5基因 原核表达 

分 类 号：S852.65[农业科学—基础兽医学]