RNAi靶向NP、L基因抑制鹅源副黏病毒在鸡胚成纤维细胞中的复制  

Inhibition of goose paramyxovirus(GPMV) replication by RNA interference targeted to matrix protein NP and L gene in CEF

作　　者：刘美[1] 母连志[1] 孟轲音[2] 丁壮[1] 丛彦龙[1] 尹仁福[1] 李志杰[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2009年第6期705-709,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571375,30771606)

摘　　要：构建了针对鹅源副黏病毒(GPMV)NA-1株NP及L基因的RNA干扰质粒;将转染质粒36 h后的鸡胚成纤维细胞接种病毒,通过病毒滴度、实时荧光定量RT-PCR、细胞病变以及间接免疫荧光结果分析,表明均能抑制GPMV在CEF中复制和增殖。本试验对研究GPMV复制和抗病毒治疗提供了技术基础。We designed plasmids that continuously expressing siRNAs targeting the GPMV NA-1 matrix protein NP and L gene. Virus titration,Real time RT-PCR,CPE and IFA indicated that NP-specific and L-specific siRNA can inhibit virus replication at 36 h post-virus infection. In future studies,a combination of siRNAs targeting the NP and L gene may be used as a tool to study NDV replication and antiviral therapy.

关 键 词：鹅源副黏病毒 RNA干扰 鸡胚成纤维细胞 抑制 

分 类 号：S852.659.5[农业科学—基础兽医学]