NQO1C_(609T),RAD51_(G135C)和XRCC3_(C241T)单核苷酸多态性与急性淋巴细胞白血病发病相关性研究  

Relationship between NQO1C_(609T),RAD51_(G135C),XRCC3_(C241T) Single Nucleotide Polymorphisms and Acute Lymphoblastic Leukemia

作　　者：张战强 杨琳 张悦 杨艺红 聂玲 李璘 王建祥 竺晓凡 肖志坚 

机构地区：[1]中国医学科学院、北京协和医学院血液学研究所、血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2009年第3期523-528,共6页Journal of Experimental Hematology

摘　　要：本研究探索NQO1C609T,RAD51G135C和XRCC3C241T单核苷酸多态性与急性淋巴细胞白血病(ALL)发生的关系。对170例ALL患者和458名与患者无血缘关系的正常人,用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法分析其NQO1C609T,RAD51G135C和XRCC3C241T基因型。结果表明:在单基因水平分析时NQO1C609T,RAD51G135C和XRCC3C241T基因型比例在正常对照和ALL患者之间无统计学差异,提示其单独作用时对ALL发病的影响无统计学意义。当3个基因联合分析时,NQO1C609T和RAD51G135C均为变异型时伴髓系抗原阳性的ALL和伴平衡易位ALL的发病风险增加(OR值分别为5.553和2.618);NQO1C609T纯合变异型时农村儿童ALL的发病风险增加(OR值为2.541)。结论:NQO1C609T、RAD51G135C和XRCC3C241T基因型联合作用可能促进ALL的发病,提示多基因联合分析较单基因对ALL的发病分析可能更有预测意义。This study was purposed to investigate the relationship between NQ01C609T,RAD51G135c,XRCC3c241T single nucleotide polymorphisms and incidence of acute lymphoblastic leukemia （ALL）. NQ01C609T,RAD51G135c,XRCC3c241T genotypes were detected by PCR-RFLP in 170 patients with de novo ALL and 458 normal persons as control. The results indicated that the genotype ratio of NQ01C609T,RAD51G135c and XRCC3c241T in single genotype analysis showed no statistical difference between ALL patients and normal controls, which suggested that the singie genotype affect onset of ALL without statistical significance. In combined genotype analysis, presence of both variants for NQO1C609T and RAD51C135c increased onset risk of ALL with myeloid antigen positive and with balanced translocation （ OR value 5. 553 and 2. 618 respectively） ; the presence of homozygosity variant for NQO1C609r increased onset risk of ALL in the country-children （ OR = 2. 541 ）. In conclusion, the combined effect of NNQ01C609T,RAD51G135c and XRCC3c241T genotypes may promote occurence of ALL, which suggests that the combined analysis of 3 genotypes has more predictive significance for ALL than single genotype analysis.

关 键 词：急性淋巴细胞白血病 NQO1基因 RAD51基因 XRCC3基因 同源重组修复 单核苷酸多态性 

分 类 号：R733.71[医药卫生—肿瘤]