新疆维吾尔族与汉族直肠癌组织APC基因突变分析  

Study on the relationship between APC gene mutations and their clinicopathologic significance in rectal cancer

作　　者：范川文[1] 库热西.玉努斯 王海江[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胃肠外科,新疆乌鲁木齐830011 [2]新疆医科大学新疆地方病分子生物学重点实验室,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2009年第5期585-587,共3页Journal of Xinjiang Medical University

摘　　要：目的:探索APC基因突变与新疆维吾尔族、汉族直肠癌的发生及临床病理之间的关系。方法:采用银染PCR-SSCP法检测新疆地区70例原发性直肠腺癌组织及距肿瘤10cm以上的正常粘膜组织中APC基因MCR之碱基突变。结果:70例直肠癌组织中APC基因突变38例(54.3%),APC基因在新疆维吾尔族和汉族直肠癌组织中的突变频率较高,分别为53.6%和57.1%,但两民族之间突变率、突变与各临床病理因素之间的差异均无统计学意义(P>0.05)。结论:新疆地区APC基因MCR突变在民族之间以及和临床病理之间均无相关性。Objective. To explore the relationship between the APC gene mutation and the clinicopathological significance in rectal cancer of the Xinjiang Uygur and Han. Methods： The mutations in mutation cluster region （MCR） of APC gene was detected by the polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） methad in 70 cases of the Xinjiang region with primary rectal adenocarcinoma and the normal mucosal tissues. Results： There was no association between clinicopathologic factors and the APC gene mutation. No statistical difference was found in different ethnic groups. Conclusion. There was no correlation between the mutation of APC gene and the clinicopathological significance in rectal carcinoma among different ethnic groups in Xinjiang region.

关 键 词：直肠癌 腺瘤性息肉病基因 基因突变 聚合酶链反应-单链构象多肽性 临床病理 

分 类 号：R735.3[医药卫生—肿瘤]