硼替佐米诱导Burkitt淋巴瘤Raji细胞凋亡的研究  

Apoptosis of Burkitt's Lymphoma Raji Cell Line Induced by Bortezomib

作　　者：杨小刚[1] 李晓林[1] 陈懿建[2] 谢兆霞[1] 彭敏源[1] 黄晓春[1] 黄然欣[1] 

机构地区：[1]中南大学湘雅医院血液科,湖南长沙410008 [2]赣南医学院第一附属医院血液科,江西赣州341000

出　　处：《中国实验血液学杂志》2009年第3期592-596,共5页Journal of Experimental Hematology

摘　　要：本研究探讨硼替佐米对Burkitt淋巴瘤Raji细胞是否具有凋亡诱导作用及其机制。以硼替佐米处理Raji细胞,观察细胞增殖抑制作用的时间效应和剂量效应,在光学显微镜下观察药物作用前后细胞的形态变化,用流式细胞术检测药物孵育前后细胞凋亡率(AP),用RT-PCR法检测药物孵育前后NF-κB和p53基因的表达水平。结果表明,在一定剂量和时间范围内硼替佐米能抑制Raji细胞生长;硼替佐米能诱导Raji细胞凋亡并显示时间和剂量效应;在硼替佐米诱导Raji细胞凋亡过程中,NF-κB及p53基因的表达水平下调。结论:硼替佐米能诱导Raji细胞凋亡,NF-κB基因和p53基因很可能参与了硼替佐米诱导Raji细胞凋亡的调控。The aim of this study was to clarify whether bortezomib might induce apoptosis in Burkitt＇s lymphoma Raji cell line and its mechanism. Different concentrations of bortezomib were used to treat Raji cells and its effects of time and dose were observed. Cell morphology was observed under light microscope; flow cytometry was used to analyze cell apoptosis ; RT-PCR was used to detect the expressions of NF-κB and p53 gene mRNAs. The results showed that the bortezomib could inhibit Raji cell growth within a certain range of treating time and dose. Apoptosis were induced in relation to time and dose. The expression of NF-κB mRNA and p53 mRNA decreased after treatment with bortezomib. It is concluded that the bortezomib can induce Raji cell apoptosis, which provides a theoretical basis for clinical treatment. NF-κB and p53 gene are supposed to participate in the bortezomib induced apoptosis of Raji cells.

关 键 词：硼替佐米 细胞凋亡 NF—κB p53 RAJI细胞 BURKITT淋巴瘤 

分 类 号：R733.1[医药卫生—肿瘤] R979.1[医药卫生—临床医学]