在不同类型骨髓增生异常综合征Id4基因甲基化差异的初步研究  

Preliminary Study on Difference of Id4 Gene Methylation in Various Types of Myelodysplastic Syndromes

作　　者：赵瑜[1] 薄剑[1] 窦丽萍[1] 靖彧[1] 王书红[1] 王全顺[1] 李红华[1] 于力[1] 

机构地区：[1]中国人民解放军总医院血液科,北京100853

出　　处：《中国实验血液学杂志》2009年第3期618-620,共3页Journal of Experimental Hematology

基　　金：973国家重点基础研究专项经费资助项目(2005CB522400);国家自然科学基金资助项目(30572108);解放军总医院苗圃基金(07MP02)

摘　　要：本研究探讨Id4基因在不同类型骨髓增生异常综合症(MDS)患者中的甲基化情况差异。采用甲基化特异性聚合酶链反应(MS-PCR)对50例不同类型MDS患者骨髓进行Id4基因启动子区甲基化状况检测,并以缺铁性贫血患者骨髓作为对照。结果发现:Id4基因在对照组骨髓中呈完全非甲基化状态,在MDS各种类型中,甲基化状态有差异:6例RA、2例RARS和4例MDS-U患者骨髓Id4基因均呈非甲基化状态,而18例RCMD中有2例,12例RAEBⅠ和8例RAEBⅡ中各有3例为Id4基因甲基化。骨髓原始细胞比例分别低于和高于5%的两组患者中,Id4甲基化分别有2例和6例,各占每组的6.7%和30%,差异有显著性意义。初步结论是:骨髓原始细胞比例高的MDS患者有可能发现Id4基因甲基化。The aim of this study was to investigate the difference of Id4 gene promoter methylation in patients with different subtypes of myelodysplastic syndromes （MDS）. By using MS-PCR method, the methylation status of Id4 gene was detected in 50 patients with different subtypes of MDS. Id4 methylation was also detected in bone marrow samples from patients with iron deficiency anemia which served as control. The results showed that Id4 gene was unmethylated in all of bone marrow samples from controls. In various subtypes of MDS patients, the rate of Id4 gene methylation was different. No It4 methylation was found in 6 cases of RA, 2 cases of RARS and 4 cases of MDS-U. Id4 methylations was found in 2 out of 18 patients with RCMD. Id4 methylation in 3 out of 12 patients with RAEB Ⅰ and other 3 out 8 patients with RAEB Ⅱ were found. In groups with blast ratio lower or higher than 5%, the incidence of Id4 gene methylation were 6.7% and 30% respectively, so that there was significant difference. In tentative conclusion, Id4 gene methylation possibly is found in MDS patients with higher ratio of blast cells.

关 键 词：ID4基因 骨髓增生异常综合症 基因甲基化 

分 类 号：R733.7[医药卫生—肿瘤] R733.3[医药卫生—临床医学]