N-乙酰半胱氨酸减轻大鼠纹状体甲基苯丙胺神经毒性  

N-Acetylcysteine attenuates methamphetamine induced neurotoxicity in rat striatum

作　　者：宋健文[1] 刘增甲[1] 谭晓辉[1] 王慧君[1] 

机构地区：[1]南方医科大学基础医学院法医系,广东广州510515

出　　处：《中国药理学通报》2009年第6期765-769,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30872918)

摘　　要：目的探讨N-乙酰半胱氨酸(NAC)对甲基苯丙胺(METH)中毒大鼠模型纹状体神经毒性的影响及作用机制。方法制备大鼠中毒模型,在METH前30min腹腔注射(ip)NAC,应用二氯荧光乙酰乙酸盐作为荧光指标检测纹状体ROS的含量,高效液相色谱方法检测DA浓度,TUNEL方法观察神经元损害情况,并计算神经元的凋亡率。结果NAC预处理能降低纹状体内ROS的含量,减轻DA浓度的下降程度,减少神经细胞的凋亡。结论NAC通过抑制METH诱导纹状体的氧化应激,减轻METH多巴胺能神经毒性。Aim To explore the effects of N-acetylcysteine （NAC） on methamphetamine （METH） induced neurotoxicity of striatum in rat model and its mechanism. Methods NAC was administrated （ip） 30 rain prior to METH injection to produce toxic rat model. Dihydrodichlorofluoreseein diacetate was used to detect the level of reactive oxygen species （ ROS）, high performance liquid chromatography for the concentration of Dopamine （ DA）, meanwhile, TUNEL method was used to observe the neuron apoptosis. Results Pretreatment with NAC could decrease the level of ROS in rat striaturn, prevent the loss of DA and decrease the TUNELpositive neurons. Conclusion NAC could attenuate methamphetamine-induced neurotoxicity in rat striatum by blocking ROS production.

关 键 词：甲基苯丙胺 神经毒性 氧化应激 N-乙酰半胱氨酸 多巴胺 凋亡 

分 类 号：R-332[医药卫生] R322.81