低聚壳聚糖Zn(Ⅱ)配合物清除DPPH活性的研究  

DPPH Scavenging Activity of Chitosan Oligosaccharide Zinc Complexes

作　　者：银旭红[1] 孙涛[1] 姚倩[1] 周冬香[1] 毛芳[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《食品与生物技术学报》2009年第3期329-331,共3页Journal of Food Science and Biotechnology

基　　金：上海市教育委员会科研项目(07zz134);上海市重点学科建设专项基金项目(T1102)

摘　　要：控制反应物低聚壳聚糖和氯化锌的比例,合成了两种金属含量不同的低聚壳聚糖锌配合物,用红外光谱对产物结构进行表征,原子吸收光谱证实两种配合物中的锌质量分数分别为5.04%和8.68%。考察了其对DPPH的清除活性,结果表明:两种配合物对DPPH有明显的清除效果,锌质量分数为5.04%和8.68%的低聚壳聚糖锌配合物对DPPH的半抑制浓度IC50分别为1.50 mg/mL和1.76 mg/mL,但均大于低聚壳聚糖对DPPH的半抑制浓度IC500.42 mg/mL,这可能是因为金属离子的引进,使部分活性氨基和羟基的数目减少所致。Two chitosan oligosaccharide zinc complexes with different zinc content were successful prepared by controlling the molar ratio of chitosan oligosaecharide to zinc chloride, the content of zinc was 5.04% and 8. 68%, respectively. The those chitosan oligosaecharide were identified by Atomic Absorption Spectrometry（AAS）, and the structure of the complexes were characterized by FTIR spectra. DPPH scavenging activity of chitosan oligosaccharide and the two ehitosan oligosaccharide metal complexes were evaluated, the result showed, the complexes have obvious effect on DPPH scavenging. The IC50 of chitosan oligosaccharide zinc （5.04%） and chitosan oligosaccharide zinc（8.68%）were 1.5 mg/mL and 1.76 mg/mL, respectively,. But both higher than chitosan oligosaccharide, the IC50 was 0.42 mg/mL. That may be related with active amino and hydroxyl both were reduced by introducing the metal ion.

关 键 词：1 1-二苯基苦基苯肼 壳聚糖 壳聚糖金属配合物 

分 类 号：R96[医药卫生—药理学]