氯氟氰菊酯降解菌的分离和培养条件研究  

Study on isolation and culture conditions of cyhalothrin degrading bacterium

作　　者：吴浩豪[1] 呼世斌[2] 陈小翠[1] 秦宝福[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100

出　　处：《中国土壤与肥料》2009年第3期71-74,共4页Soil and Fertilizer Sciences in China

基　　金：中央环保专项基金(财建[2006]859号)

摘　　要：从某农药厂排污口的污泥中采集样品，经驯化富集后筛选到1株能高效降解氯氟氰菊酯的细菌X2。在30℃，pH7．0基础培养基中，该菌在7d内对浓度为200mg／L的氯氟氰菊酯的降解率为77．2％。依据生理生化特征及16SrDNA测定分析，将该菌株确定为鞘氨醇杆菌属（Sphingobacterium sp．）。降解特性研究表明，在温度25—30℃，pH为7．0—8．0的条件下，该菌株能有效降解氯氟氰菊酯，因而可以将其应用于氯氟氰菊酯污染土壤的生物修复。A bacterium X2 capable of utilizing cyhalothrin as sole carbon source was isolated from wastewater treating system of a pesticide manufacturer. For the optimal pH and temperature for the degradation were 7.0 and 30℃, respectively, this bacterium could degrade 77. 2% of 200 mg/L eyhalothrin in 7 days. This bacterium was identified as Sphingobacterium sp. according to its physiological ＆ biochemical analysis and the similarity analysis of its 16S rDNA sequence. The cyhalothrin could be degraded over a temperature range of 25-30℃ and pH range 7.0-8.0. The results indicated that strain X2 had potential use in bioremediation of cyhalothrin contaminated soil.

关 键 词：氯氟氰菊酯 生物降解 降解菌 

分 类 号：X172[环境科学与工程—环境科学]