牛磺酸对离体大鼠脊髓缺氧/复氧损伤的保护作用  

Experimental Study of Taurine Protective Effect on Hypoxia/ Reoxygenation in Isolated Rat Spinal Cord

作　　者：王吉先[1] 姚兴海[1] 王立智 李燕[1] 苏静怡[1] 唐朝枢[1] 

机构地区：[1]北京医科大学心血管基础研究所

出　　处：《基础医学与临床》1998年第3期57-60,56,共5页Basic and Clinical Medicine

摘　　要：本文在孵育的离体大鼠脊髓组织切片上，观察牛磺酸对缺氧／复氧损伤的影响。缺氧／复氧损伤引起大鼠脊髓组织ＡＴＰ含量减少和ＬＤＨ漏出增多，ＮＯ生成和ＮＯＳ活性明显增加。Ｌ精氨酸转运Ｖｍａｘ增加９７％（Ｐ＜００１），Ｋｍ值无明显变化。用１０和２０ｍｍｏｌ／Ｌ牛磺酸孵育与单纯缺氧／复氧组比较，显著减轻了缺氧／复氧引起的组织ＡＴＰ减少和ＬＤＨ漏出，ＮＯ生成分别低３４％（Ｐ＜００１）和４０％（Ｐ＜００１），组织Ｌ精氨酸转运Ｖｍａｘ降低３２％（Ｐ＜００１）和３８％（Ｐ＜００１），但仅轻度抑制ＮＯＳ活性增加。提示牛磺酸对Ｌ精氨酸／一氧化氮系统的影响可能是其对缺氧／复氧损伤的神经保护作用的机理之一。The effect of taurine on hypoxia/reoxygenation(H/R) injury in tissue slices of isolated rat spinal cord was investigated.There are decrease of ATP content,increase of LDH leakage,No production and NO Synthase(NOS)activity were obviously elevated during H/R.Maximum transport velocity(Vmax)of L Arginine(L Arg)is 97% higher(P<0 01),while Michaelis Constant(Km)showed no significant changes.In comparison with H/R group,10 and 20 mmol taurine could significantly alleviate ATP decrease and LDH leakage;NO production reduced 34% (P<0 01) and 40%(P<0 01)respectively.Vmax of L Arg lowered 32%(P<0 01) and 38%(P<0 01).But NOS activity was slightly inhibited.Thus it might be suggested that effect of taurine on L Arg /NO sytem may be one of the nervous protective mechanisms during H/R injury.

关 键 词：脊髓损伤 缺氧 复氧 牛磺酸 保护作用 

分 类 号：R651.2[医药卫生—外科学] R977.4[医药卫生—临床医学]